Full genome characterization of novel DS-1-like G9P[8] rotavirus strains that have emerged in Thailand

• Published in: PloS one 2020-04, Vol.15 (4), p.e0231099-e0231099
• Main Authors: Fukuda, Saori, Tacharoenmuang, Ratana, Guntapong, Ratigorn, Upachai, Sompong, Singchai, Phakapun, Ide, Tomihiko, Hatazawa, Riona, Sutthiwarakom, Karun, Kongjorn, Santip, Onvimala, Napa, Ruchusatsawast, Kriangsak, Rungnopakun, Pimpa, Mekmallika, Jutarat, Kawamura, Yoshiki, Motomura, Kazushi, Tatsumi, Masashi, Takeda, Naokazu, Murata, Takayuki, Yoshikawa, Tetsushi, Uppapong, Ballang, Taniguchi, Koki, Komoto, Satoshi
• Format: Article
• Language: English
• Subjects: Adulyadej, Bhumibol; Biology and Life Sciences; Collaboration; Computer and Information Sciences; Constellations; Genes; Genomes; Genomic analysis; Genotype & phenotype; Genotypes; Health surveillance; Hospitals; Infections; Medicine; Parasitology; Pediatrics; People and Places; Phylogeny; Proteins; Public health; Research and Analysis Methods; Rotavirus; Strains (organisms); Virology; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0231099

The emergence and rapid spread of unusual DS-1-like intergenogroup reassortant rotaviruses having G1/3/8 genotypes have been recently reported from major parts of the world (Africa, Asia, Australia, Europe, and the Americas). During rotavirus surveillance in Thailand, three novel intergenogroup reassortant strains possessing the G9P[8] genotype (DBM2017-016, DBM2017-203, and DBM2018-291) were identified in three stool specimens from diarrheic children. In the present study, we determined and analyzed the full genomes of these three strains. On full-genomic analysis, all three strains were found to share a unique genotype constellation comprising both genogroup 1 and 2 genes: G9-P[8]-I2-R2-C2-M2-A2-N2-T2-E2-H2. Phylogenetic analysis demonstrated that each of the 11 genes of the three strains was closely related to that of emerging DS-1-like intergenogroup reassortant, human, and/or locally circulating human strains. Thus, the three strains were suggested to be multiple reassortants that had acquired the G9-VP7 genes from co-circulating Wa-like G9P[8] rotaviruses in the genetic background of DS-1-like intergenogroup reassortant (likely equine-like G3P[8]) strains. To our knowledge, this is the first description of emerging DS-1-like intergenogroup reassortant strains having the G9P[8] genotype. Our observations will add to the growing insights into the dynamic evolution of emerging DS-1-like intergenogroup reassortant rotaviruses through reassortment.