Interleukin-10 control of pre-miR155 maturation involves CELF2

The anti-inflammatory cytokine interleukin-10 (IL10) is essential for attenuating inflammatory responses, which includes reducing the expression of pro-inflammatory microRNA-155 (miR155) in lipopolysaccharide (LPS) activated macrophages. miR155 enhances the expression of pro-inflammatory cytokines s...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0231639-e0231639
Main Authors: Yoon, Jeff S J, Wu, Mike K, Zhu, Tian Hao, Zhao, Helen, Cheung, Sylvia T, Chamberlain, Thomas C, Mui, Alice L-F
Format: Article
Language:English
Subjects:
Biochemistry
Biology and life sciences
CRISPR
Cytokines
Gene expression
Infections
Inflammation
Inositol-1,4,5-trisphosphate 5-phosphatase
Interleukin 1
Interleukin 10
Lipopolysaccharides
Macrophages
Maturation
Medical research
Medicine and Health Sciences
MicroRNAs
miRNA
Molecular biology
Pathogens
Phenols
Proteins
Research and Analysis Methods
Ribonucleic acid
RNA
RNA-binding protein
Surgery
Tumor necrosis factor-α
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Summary:The anti-inflammatory cytokine interleukin-10 (IL10) is essential for attenuating inflammatory responses, which includes reducing the expression of pro-inflammatory microRNA-155 (miR155) in lipopolysaccharide (LPS) activated macrophages. miR155 enhances the expression of pro-inflammatory cytokines such as TNFα and suppresses expression of anti-inflammatory molecules such as SHIP1 and SOCS1. We previously found that IL10 interfered with the maturation of pre-miR155 to miR155. To understand the mechanism by which IL10 interferes with pre-miR155 maturation we isolated proteins that associate with pre-miR155 in response to IL10 in macrophages. We identified CELF2, a member of the CUGBP, ELAV-Like Family (CELF) family of RNA binding proteins, as protein whose association with pre-miR155 increased in IL10 treated cells. CRISPR-Cas9 mediated knockdown of CELF2 impaired IL10's ability to inhibit both miR155 expression and TNFα expression.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0231639