Expression of lamin C2 in mammalian oocytes

Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown...

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Bibliographic Details
Published in:PloS one 2020-04, Vol.15 (4), p.e0229781-e0229781
Main Authors: Koncicka, Marketa, Cervenka, Jakub, Jahn, Daniel, Sucha, Rita, Vodicka, Petr, Gad, Ahmed, Alsheimer, Manfred, Susor, Andrej
Format: Article
Language:English
Subjects:
Biochemistry
Biology and Life Sciences
C2 protein
Chromosomes
Defects
Developmental biology
Gametocytes
Gene expression
Genes
Genetic research
Germ cells
Immunoblotting
Laboratories
Localization
Mammals
Medicine and Health Sciences
Meiosis
Molecular biology
Oocytes
Physiology
Plasmids
Proteins
Research and Analysis Methods
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Summary:Lamin C2 (LMN C2) is a short product of the lamin a gene. It is a germ cell-specific lamin and has been extensively studied in male germ cells. In this study, we focussed on the expression and localization of LMN C2 in fully-grown germinal vesicle (GV) oocytes. We detected LMN C2 in the fully-grown germinal vesicle oocytes of various mammalian species with confirmation done by immunoblotting the wild type and Lmnc2 gene deleted testes. Expression of LMN C2 tagged with GFP showed localization of LMN C2 to the nuclear membrane of the oocyte. Moreover, the LMN C2 protein notably disappeared after nuclear envelope breakdown (NEBD) and the expression of LMN C2 was significantly reduced in the oocytes from aged females and ceased altogether during meiotic maturation. These results provide new insights regarding LMN C2 expression in the oocytes of various mammalian species.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0229781