Comprehensive proteomics and functional annotation of mouse brown adipose tissue

• Published in: PloS one 2020-05, Vol.15 (5), p.e0232084-e0232084
• Main Authors: Li, Jing, Li, Juan, Zhao, Wei-Gang, Sun, Hai-Dan, Guo, Zheng-Guang, Liu, Xiao-Yan, Tang, Xiao-Yue, She, Zhu-Fang, Yuan, Tao, Liu, Shuai-Nan, Liu, Quan, Fu, Yong, Sun, Wei
• Format: Article
• Language: English
• Subjects: Adenosine; Adipocytes; Adipose tissue; Adipose tissue (brown); Adipose Tissue, Brown - chemistry; Adipose Tissue, Brown - metabolism; Animal tissues; Animals; Annotations; Apoptosis; Biology and Life Sciences; Biomarkers; Body fat; Chromatography; Chromatography, High Pressure Liquid; Chromatography, Liquid; Cytokines; Databases, Protein; Diabetes; Endocrinology; Fatty acids; Female; Future predictions; Glucose; Hospitals; House mouse; Kinases; Laboratories; Mammals; Mass spectrometry; Mass spectroscopy; Medical schools; Medicine; Medicine and Health Sciences; Methods; Mice; Mice, Inbred C57BL; Molecular modelling; Molecular Sequence Annotation - methods; Oxidation; Oxidation-reduction reactions; Peptides; Phosphorylation; Physiological aspects; Protein biosynthesis; Protein synthesis; Proteins; Proteome - analysis; Proteome - metabolism; Proteomes; Proteomics; Proteomics - methods; R&D; Research & development; Scientific imaging; Signal peptides; Spectroscopy; Studies; Tandem Mass Spectrometry; Thermogenesis; Thermoregulation; Time; Type 2 diabetes
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0232084

Knowledge about the mouse brown adipose tissue (BAT) proteome can provide a deeper understanding of the function of mammalian BAT. Herein, a comprehensive analysis of interscapular BAT from C57BL/6J female mice was conducted by 2DLC and high-resolution mass spectrometry to construct a comprehensive proteome dataset of mouse BAT proteins. A total of 4949 nonredundant proteins were identified, and 4495 were quantified using the iBAQ method. According to the iBAQ values, the BAT proteome was divided into high-, middle- and low-abundance proteins. The functions of the high-abundance proteins were mainly related to glucose and fatty acid oxidation to produce heat for thermoregulation, while the functions of the middle- and low-abundance proteins were mainly related to protein synthesis and apoptosis, respectively. Additionally, 497 proteins were predicted to have signal peptides using SignalP4 software, and 75 were confirmed in previous studies. This study, for the first time, comprehensively profiled and functionally annotated the BAT proteome. This study will be helpful for future studies focused on biomarker identification and BAT molecular mechanisms.