Mycelial biomass estimation and metabolic quotient of Lentinula edodes using species-specific qPCR

Lentinula edodes, commonly known as shiitake, is an edible mushroom that is cultivated and consumed around the globe, especially in Asia. Monitoring mycelial growth inside a woody substrate is difficult, but it is essential for effective management of mushroom cultivation. Mycelial biomass also affe...

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Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0232049-e0232049
Main Authors: Jomura, Mayuko, Kuwayama, Tomoko, Soma, Yuto, Yamaguchi, Muneyoshi, Komatsu, Masabumi, Maruyama, Yutaka
Format: Article
Language:English
Subjects:
Basidiocarps
Biology and Life Sciences
Biomass
Calibration
Cultivation
Decomposition
Deoxyribonucleic acid
DNA
Dust
Ecology and Environmental Sciences
EDTA
Environmental conditions
Forest products
Fungi
Fungiculture
Genetic testing
Growth
Lentinula edodes
Measurement
Metabolism
Mushrooms
Mycelia
Mycelium
Plant biomass
Plant species
Quotients
Research and Analysis Methods
Shiitake
Species
Substrates
Wood
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Summary:Lentinula edodes, commonly known as shiitake, is an edible mushroom that is cultivated and consumed around the globe, especially in Asia. Monitoring mycelial growth inside a woody substrate is difficult, but it is essential for effective management of mushroom cultivation. Mycelial biomass also affects the rate of wood decomposition under natural conditions and must be known to determine the metabolic quotient, an important ecophysiological parameter of fungal growth. Therefore, developing a method to measure it inside a substrate would be very useful. In this study, as the first step in understanding species-specific rates of fungal decomposition of wood, we developed species-specific primers and qPCR procedures for L. edodes. We tested primer specificity using strains of L. edodes from Japan and Southeast Asia, as well as related species of fungi and plant species for cultivation of L. edodes, and generated a calibration curve for quantification of mycelial biomass in wood dust inoculated with L. edodes. The qPCR procedure we developed can specifically detect L. edodes and allowed us to quantify the increase in L. edodes biomass in wood dust substrate and calculate the metabolic quotient based on the mycelial biomass and respiration rate. Development of a species-specific method for biomass quantification will be useful for both estimation of mycelial biomass and determining the kinetics of fungal growth in decomposition processes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0232049