Proteomics of intracellular freezing survival

Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P....

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Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0233048-e0233048
Main Authors: Thorne, Michael A S, Britovšek, Nina Kočevar, Hawkins, Liam, Lilley, Kathryn S, Storey, Kenneth
Format: Article
Language:English
Subjects:
Acclimation
Acclimatization
Acclimatization (Biology)
Annotations
Antioxidants
Antioxidants (Nutrients)
Biochemistry
Biological research
Biology and Life Sciences
Cryobiology
Cytoskeleton
Dehydration
Environmental aspects
Freezing
Intracellular
Mass spectrometry
Methods
Nematodes
Physical Sciences
Physiological aspects
Proteases
Proteins
Proteomes
Proteomics
Research and Analysis Methods
Roundworms
Scientific imaging
Survival
Trehalose
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Summary:Panagrolaimus sp. DAW1, a nematode cultured from the Antarctic, has the extraordinary physiological ability to survive total intracellular freezing throughout all of its compartments. While a few other organisms, all nematodes, have subsequently also been found to survive freezing in this manner, P. sp. DAW1 has so far shown the highest survival rates. In addition, P. sp. DAW1 is also, depending on the rate or extent of freezing, able to undergo cryoprotective dehydration. In this study, the proteome of P. sp DAW1 is explored, highlighting a number of differentially expressed proteins and pathways that occur when the nematodes undergo intracellular freezing. Among the strongest signals after being frozen is an upregulation of proteases and the downregulation of cytoskeletal and antioxidant activity, the latter possibly accumulated before freezing much in the way the sugar trehalose has been shown to be stored during acclimation.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0233048