A simplified method for blood feeding, oral infection, and saliva collection of the dengue vector mosquitoes

A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the a...

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Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0233618-e0233618
Main Authors: Sri-In, Chalida, Weng, Shih-Che, Shiao, Shin-Hong, Tu, Wu-Chun
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Aedes - physiology
Aedes - virology
Aedes aegypti
Animal Feed - analysis
Animals
Biology and Life Sciences
Blood
Blood meals
Blood substitutes
Collection
Cotton
Culicidae
Dengue
Dengue - transmission
Dengue fever
Dengue Virus - isolation & purification
Diagnostic specimens
Eggs
Entomology
Experiments
Feeds
Female
Females
Food and nutrition
Glucose
Humans
Humidity
Infections
Insect carriers of disease
Laboratories
Meals
Medical research
Medicine
Medicine and Health Sciences
Mice
Mosquito Vectors - physiology
Mosquito Vectors - virology
Mosquitoes
Oral infection
Parasitological research
Parasitology
Pathogens
Physical Sciences
Physiological aspects
Plaque assay
Research and Analysis Methods
Saliva
Saliva - virology
Vector-borne diseases
Viruses
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Description
Summary:A simple device using folded Parafilm-M as an artificial blood feeder was designed for studying two important dengue vector mosquitoes, Aedes aegypti and Aedes albopictus. The efficiency of the artificial blood feeder was investigated by comparing the numbers of engorged mosquitoes that fed on the artificial blood feeder versus mice as a live blood source. Significantly more engorged females Aedes aegypti fed on the artificial blood feeder than on mice. In addition, the artificial feeder could serve as a useful apparatus for oral infection via artificial blood meals, and for saliva collection in mosquitoes. Our method enabled us to collect saliva from multiple mosquitoes at once, providing sufficient infected saliva for determination of the virus titer by plaque assay analysis. Our artificial feeder has the advantage that it is simple, inexpensive, and efficient.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0233618