Sequencing B cell receptors from ferrets (Mustela putorius furo)

The domestic ferret (Mustela putorius furo) provides a critical animal model to study human respiratory diseases. However immunological insights are restricted due to a lack of ferret-specific reagents and limited genetic information about ferret B and T cell receptors. Here, variable, diversity and...

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Bibliographic Details
Published in:PloS one 2020-05, Vol.15 (5), p.e0233794-e0233794
Main Authors: Wong, Julius, Tai, Celeste M, Hurt, Aeron C, Tan, Hyon-Xhi, Kent, Stephen J, Wheatley, Adam K
Format: Article
Language:English
Subjects:
Animal models
Antibodies
Antigens
B cells
Biology and Life Sciences
Cell receptors
Chains
Cloning
Domestic animals
Expression vectors
Ferrets
Gene sequencing
Genes
Genomics
Immunoglobulins
Immunology
Infections
Influenza
Interrogation
Lymphocytes
Lymphocytes B
Lymphocytes T
Mammals
Medicine and Health Sciences
Monoclonal antibodies
Mustela putorius furo
Pathogenesis
Phylogenetics
Physiological aspects
Polymerase chain reaction
Reagents
Receptors
Research and Analysis Methods
Respiratory diseases
Viral diseases
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Summary:The domestic ferret (Mustela putorius furo) provides a critical animal model to study human respiratory diseases. However immunological insights are restricted due to a lack of ferret-specific reagents and limited genetic information about ferret B and T cell receptors. Here, variable, diversity and joining genes within the ferret kappa, lambda and heavy chain immunoglobulin loci were annotated using available genomic information. A multiplex PCR approach was derived that facilitated the recovery of paired heavy and light chain immunoglobulin sequences from single sorted ferret B cells, allowing validation of predicted germline gene sequences and the identification of putative novel germlines. Eukaryotic expression vectors were developed that enabled the generation of recombinant ferret monoclonal antibodies. This work advances the ferret as an informative immunological model for viral diseases by allowing the in-depth interrogation of antibody-based immunity.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0233794