Using advanced vibrational molecular spectroscopy to detect moist heating induced protein structure changes in cool-climate adapted barley grain

Different techniques have been applied in feed processing to improve ruminal degradation and nutrient utilization in ruminant. There are limited studies investigating how moist heating process impacts barley protein utilization and internal molecular structures. The objectives of this study were to...

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Bibliographic Details
Published in:PloS one 2020-06, Vol.15 (6), p.e0234126-e0234126
Main Authors: Feng, Xin, Sun, Baoli, Zhang, Huihua, Yu, Peiqiang
Format: Article
Language:English
Subjects:
Acidosis
Analytical chemistry
Animal lactation
Barley
Biodegradation
Biology and Life Sciences
Carbohydrates
Chemical bonds
Climate change
Degradation
Engineering and Technology
Environmental aspects
Feed processing
Feeds
Fermentation
Fourier transforms
Grain
Heating
Infrared spectroscopy
Intestine
Life sciences
Medicine and Health Sciences
Methods
Milk
Moisture
Molecular spectroscopy
Molecular structure
Nutrient utilization
Nutrients
Observations
Parameters
Physical Sciences
Physiological aspects
Poultry
Protein structure
Proteins
Reflectance
Rumen
Spectra
Spectroscopy
Spectrum analysis
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Summary:Different techniques have been applied in feed processing to improve ruminal degradation and nutrient utilization in ruminant. There are limited studies investigating how moist heating process impacts barley protein utilization and internal molecular structures. The objectives of this study were to investigate: 1) how moist heating affects barley protein chemical profiles and Cornell Net Carbohydrate and Protein System (CNCPS) subfractions, in situ rumen degradation parameters, and predicted intestinal protein supply and feed milk value; 2) how moist heating affects protein molecular spectral features; and 3) the relationship between protein molecular structure spectral features and protein chemical profiles and metabolic characteristics. The barley variety CDC cowboy samples collected from the research farm during two consecutive years were used. Half of each sample was kept as raw and the other half underwent moist heating. The advanced molecular spectroscopy (attenuated total reflectance-fourier transform infrared, ATR-FTIR) was used to detect the barley protein molecular structure spectral features. It was found that moist heating decreased the fractions of soluble protein and increased the moderately degradable protein and ingestible protein fractions. This further resulted in the changes of in situ rumen degradation parameters and intestinal protein digestion characteristics. The protein molecular structure spectral features detected by using ATR-FTIR spectroscopy can be used as potential predictors for protein related chemical and metabolic parameters.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0234126