Lipidomics of human adipose tissue reveals diversity between body areas

Adipose tissue plays a pivotal role in storing excess fat and its composition reflects the history of person's lifestyle and metabolic health. Broad profiling of lipids with mass spectrometry has potential for uncovering new knowledge on the pathology of obesity, metabolic syndrome, diabetes an...

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Published in:PloS one 2020-06, Vol.15 (6), p.e0228521
Main Authors: Al-Sari, Naba, Suvitaival, Tommi, Mattila, Ismo, Ali, Ashfaq, Ahonen, Linda, Trost, Kajetan, Henriksen, Trine Foged, Pociot, Flemming, Dragsted, Lars Ove, Legido-Quigley, Cristina
Format: Article
Language:English
Subjects:
Abdomen
Adipocytes
Adipose tissue
Adipose Tissue - metabolism
Adipose Tissue - pathology
Biochemistry
Biology and Life Sciences
Biopsy
Body fat
Breast
Chromatography
Clinical medicine
Composition
Diabetes
Diabetes mellitus
Dilution
Disease
Health aspects
High performance liquid chromatography
Humans
Identification and classification
Levels
Lipid composition
Lipid metabolism
Lipidomics
Lipids
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Metabolic disorders
Metabolic syndrome
Methods
Optimization
Organ Specificity
Physical Sciences
Physiological aspects
Physiological research
Quadrupoles
Sample preparation
Scientific imaging
Spectroscopy
Thigh
Triglycerides
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cited_by cdi_FETCH-LOGICAL-c692t-1804f5f5477735b584585a33b2f4a931a9d1b361afadff6261bdd353f51d4a5f3
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creator Al-Sari, Naba
Suvitaival, Tommi
Mattila, Ismo
Ali, Ashfaq
Ahonen, Linda
Trost, Kajetan
Henriksen, Trine Foged
Pociot, Flemming
Dragsted, Lars Ove
Legido-Quigley, Cristina
description Adipose tissue plays a pivotal role in storing excess fat and its composition reflects the history of person's lifestyle and metabolic health. Broad profiling of lipids with mass spectrometry has potential for uncovering new knowledge on the pathology of obesity, metabolic syndrome, diabetes and other related conditions. Here, we developed a lipidomic method for analyzing human subcutaneous adipose biopsies. We applied the method to four body areas to understand the differences in lipid composition between these areas. Adipose tissue biopsies from 10 participants were analyzed using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. The sample preparation optimization included the optimization of the lipid extraction, the sample amount and the sample dilution factor to detect lipids in an appropriate concentration range. Lipidomic analyses were performed for adipose tissue collected from the abdomen, breast, thigh and lower back. Differences in lipid levels between tissues were visualized with heatmaps. Lipidomic analysis on human adipose biopsies lead to the identification of 186lipids in 2 mg of sample. Technical variation of the lipid-class specific internal standards were below 5%, thus indicating acceptable repeatability. Triacylglycerols were highly represented in the adipose tissue samples, and lipids from 13 lipid classes were identified. Long polyunsaturated triacylglycerols in higher levels in thigh (q
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subjects Abdomen
Adipocytes
Adipose tissue
Adipose Tissue - metabolism
Adipose Tissue - pathology
Biochemistry
Biology and Life Sciences
Biopsy
Body fat
Breast
Chromatography
Clinical medicine
Composition
Diabetes
Diabetes mellitus
Dilution
Disease
Health aspects
High performance liquid chromatography
Humans
Identification and classification
Levels
Lipid composition
Lipid metabolism
Lipidomics
Lipids
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medicine and Health Sciences
Metabolic disorders
Metabolic syndrome
Methods
Optimization
Organ Specificity
Physical Sciences
Physiological aspects
Physiological research
Quadrupoles
Sample preparation
Scientific imaging
Spectroscopy
Thigh
Triglycerides
title Lipidomics of human adipose tissue reveals diversity between body areas
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