Adhesion of streptococci to titanium and zirconia

• Published in: PloS one 2020-06, Vol.15 (6), p.e0234524-e0234524
• Main Authors: Oda, Yukari, Miura, Tadashi, Mori, Gentaro, Sasaki, Hodaka, Ito, Taichi, Yoshinari, Masao, Yajima, Yasutomo
• Format: Article
• Language: English
• Subjects: Adhesion; Bacteria; Bacterial adhesion; Biofilms; Biology and Life Sciences; Cell adhesion; Cell adhesion & migration; Contact angle; Dental plaque; Disks; Mathematics; Medicine and Health Sciences; Metal surfaces; Oral hygiene; Photoelectron spectroscopy; Photoelectrons; Physical Sciences; Physiological aspects; Polycrystals; Properties; Research and Analysis Methods; Scanning electron microscopy; Spectroscopy; Streptococcus; Streptococcus infections; Surface roughness; Testing; Tetragonal zirconia; Tetragonal zirconia polycrystals; Titanium; Transplants & implants; Wettability; X ray photoelectron spectroscopy; Yttria-stabilized zirconia; Yttrium oxide; Zirconia; Zirconium; Zirconium dioxide
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0234524

The purpose of this study was to evaluate the adherence of streptococci to disks of titanium (commercially pure titanium: CpTi) and zirconia (tetragonal zirconia polycrystals: TZP). CpTi and yttria-stabilized TZP disks with a mirror-polished surface were used as specimens. The arithmetic mean surface roughness (Ra and Sa) and the surface wettability of the experimental specimens were measured. For analyzing the outermost layer of the experimental specimens, X-ray photoelectron spectroscopy (XPS) analysis was performed. Streptococcus sanguinis, S. gordonii, S. oralis, and S. mutans were used as streptococcal bacterial strains. These bacterial cultures were grown for 24 h on CpTi and TZP. The number of bacterial adhesions was estimated using an ATP-bioluminescent assay, and scanning electron microscope (SEM) observation of the adhered bacterial specimens was performed. No significant differences in surface roughness or wettability were found between CpTi and TZP. In XPS analyses, outermost layer of CpTi included Ti0 and Ti.sup.4+, and outermost layer of TZP included Zr.sup.4+ . In the cell adhesion assay, the adherences of S. sanguinis, S. gordonii, and S. oralis to TZP were significantly lower than those to CpTi (p < 0.05); however, significant difference was not observed for S. mutans among the specimens. The adherence to CpTi and TZP of S. mutans was significantly lower than that of S. sanguinis, S. gordonii, and S. oralis. These results were confirmed by SEM. S. sanguinis, S. gordonii, and S. oralis adhered less to TZP than to CpTi, but the adherence of S. mutans was similar to both surfaces. S. mutans was less adherent compare with the other streptococci tested in those specimens.