Association of smoking habits with TXNIP DNA methylation levels in leukocytes among general Japanese population

Thioredoxin-interacting protein (TXNIP) inhibits the activity of thioredoxin (TXN), leading to increased oxidative stress. Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determi...

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Published in:PloS one 2020-07, Vol.15 (7), p.e0235486-e0235486
Main Authors: Maeda, Keisuke, Yamada, Hiroya, Munetsuna, Eiji, Fujii, Ryosuke, Yamazaki, Mirai, Ando, Yoshitaka, Mizuno, Genki, Ishikawa, Hiroaki, Ohashi, Koji, Tsuboi, Yoshiki, Hashimoto, Shuji, Hamajima, Nobuyuki, Suzuki, Koji
Format: Article
Language:English
Subjects:
Biology and life sciences
Cancer
Cigarettes
Deoxyribonucleic acid
DNA
DNA methylation
DNA sequencing
Gene expression
Genetic aspects
Glucose
Health aspects
Leukocytes
Levels
Lifestyles
Medicine
Medicine and Health Sciences
Metabolism
Methods
Oxidative stress
Proteins
Questionnaires
Regression analysis
Smoking
Social Sciences
Studies
Thioredoxin
Variance analysis
White blood cells
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creator Maeda, Keisuke
Yamada, Hiroya
Munetsuna, Eiji
Fujii, Ryosuke
Yamazaki, Mirai
Ando, Yoshitaka
Mizuno, Genki
Ishikawa, Hiroaki
Ohashi, Koji
Tsuboi, Yoshiki
Hashimoto, Shuji
Hamajima, Nobuyuki
Suzuki, Koji
description Thioredoxin-interacting protein (TXNIP) inhibits the activity of thioredoxin (TXN), leading to increased oxidative stress. Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determine the association between smoking habits and TXNIP DNA methylation levels in a Japanese population. We conducted a cross-sectional study of 417 subjects (180 males and 237 females) participating in a health examination. We used a pyrosequencing assay to determine TXNIP DNA methylation levels in leukocytes. The mean TXNIP DNA methylation level in current smokers (75.3%) was significantly lower than that in never and ex-smokers (never: 78.1%, p < 0.001; ex: 76.9%, p = 0.013). Multivariable logistic regression analyses showed that the OR for TXNIP DNA hypomethylation was significantly higher in current smokers than that in never smokers, and significantly higher in current smokers with years of smoking [greater than or equal to] 35 and Brinkman Index [greater than or equal to] 600 compared to that in non-smokers. In conclusion, we found that current smokers had TXNIP DNA hypomethylation compared to never and ex-smokers. Moreover, long-term smoking and high smoking exposure also were associated with TXNIP DNA hypomethylation.
doi_str_mv 10.1371/journal.pone.0235486
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Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determine the association between smoking habits and TXNIP DNA methylation levels in a Japanese population. We conducted a cross-sectional study of 417 subjects (180 males and 237 females) participating in a health examination. We used a pyrosequencing assay to determine TXNIP DNA methylation levels in leukocytes. The mean TXNIP DNA methylation level in current smokers (75.3%) was significantly lower than that in never and ex-smokers (never: 78.1%, p &lt; 0.001; ex: 76.9%, p = 0.013). Multivariable logistic regression analyses showed that the OR for TXNIP DNA hypomethylation was significantly higher in current smokers than that in never smokers, and significantly higher in current smokers with years of smoking [greater than or equal to] 35 and Brinkman Index [greater than or equal to] 600 compared to that in non-smokers. In conclusion, we found that current smokers had TXNIP DNA hypomethylation compared to never and ex-smokers. 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Expression of the TXNIP gene is regulated by DNA methylation. However, no study has reported the influence of lifestyle factors on TXNIP DNA methylation. Our goal was to determine the association between smoking habits and TXNIP DNA methylation levels in a Japanese population. We conducted a cross-sectional study of 417 subjects (180 males and 237 females) participating in a health examination. We used a pyrosequencing assay to determine TXNIP DNA methylation levels in leukocytes. The mean TXNIP DNA methylation level in current smokers (75.3%) was significantly lower than that in never and ex-smokers (never: 78.1%, p &lt; 0.001; ex: 76.9%, p = 0.013). Multivariable logistic regression analyses showed that the OR for TXNIP DNA hypomethylation was significantly higher in current smokers than that in never smokers, and significantly higher in current smokers with years of smoking [greater than or equal to] 35 and Brinkman Index [greater than or equal to] 600 compared to that in non-smokers. In conclusion, we found that current smokers had TXNIP DNA hypomethylation compared to never and ex-smokers. Moreover, long-term smoking and high smoking exposure also were associated with TXNIP DNA hypomethylation.</abstract><cop>San Francisco</cop><pub>Public Library of Science</pub><pmid>32609762</pmid><doi>10.1371/journal.pone.0235486</doi><tpages>e0235486</tpages><orcidid>https://orcid.org/0000-0003-1730-2059</orcidid><orcidid>https://orcid.org/0000-0002-3235-9558</orcidid><oa>free_for_read</oa></addata></record>
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1932-6203
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subjects Biology and life sciences
Cancer
Cigarettes
Deoxyribonucleic acid
DNA
DNA methylation
DNA sequencing
Gene expression
Genetic aspects
Glucose
Health aspects
Leukocytes
Levels
Lifestyles
Medicine
Medicine and Health Sciences
Metabolism
Methods
Oxidative stress
Proteins
Questionnaires
Regression analysis
Smoking
Social Sciences
Studies
Thioredoxin
Variance analysis
White blood cells
title Association of smoking habits with TXNIP DNA methylation levels in leukocytes among general Japanese population
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T14%3A56%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20of%20smoking%20habits%20with%20TXNIP%20DNA%20methylation%20levels%20in%20leukocytes%20among%20general%20Japanese%20population&rft.jtitle=PloS%20one&rft.au=Maeda,%20Keisuke&rft.date=2020-07-01&rft.volume=15&rft.issue=7&rft.spage=e0235486&rft.epage=e0235486&rft.pages=e0235486-e0235486&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0235486&rft_dat=%3Cgale_plos_%3EA628243398%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c5846-34f69d452d090b1a2b6db9fc73f4aafa239a30176cc1dfa4988d7b95706b1d33%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2419347935&rft_id=info:pmid/32609762&rft_galeid=A628243398&rfr_iscdi=true