Growing up in Ancient Sardinia: Infant-toddler dietary changes revealed by the novel use of hydrogen isotopes (δ2H)

• Published in: PloS one 2020-07, Vol.15 (7), p.e0235080-e0235080
• Main Authors: Ryan, Saskia E, Reynard, Linda M, Pompianu, Elisa, van Dommelen, Peter, Murgia, Clizia, Subirà, M. Eulàlia, Tuross, Noreen
• Format: Article
• Language: English
• Subjects: Age; Ancient civilizations; Anthropological research; Archaeology; Babies; Barley; Biology and Life Sciences; Birth; Breast feeding; Breastfeeding & lactation; Cemeteries; Child nutrition; Children; Collagen; Collagens; Dentin; Diet; Evolutionary biology; Food; Hydrogen; Hydrogen ion concentration; Hydrogen isotopes; Infant mortality; Isotopes; Lipids; Measurement; Medicine and Health Sciences; Molars; People and Places; Physical Sciences; Social aspects; Studies; Teeth; Weaning
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0235080

Detailed information about the lives and deaths of children in antiquity is often in short supply. Childhood dietary histories are, however, recorded and maintained in the teeth of both juveniles and adults. Primary tooth dentinal collagen does not turn over, preserving a sequential record of dietary changes. The use of nitrogen ([delta].sup.15 N) and carbon ([delta].sup.13 C) isotope values of incrementally sampled dentin are used in the study of breastfeeding practices but evidence for the addition of weaning foods, both in terms of mode and, particularly, duration, has remained analytically inaccessible to date. Here, we demonstrate how the novel use hydrogen isotope ([delta].sup.2 H) values of sequentially micro-sampled dentin collagen, measured from individuals excavated from a Punic cemetery, in Sardinia, Italy, can serve as a proxy for weaning food type and duration in ancient childhood diet. The weaning rate and age, based on the decline in [delta].sup.15 N and [delta].sup.13 C values of permanent first molars and the concomitant increase in [delta].sup.2 H, appears to be broadly similar among six individuals. Hydrogen isotopes vary systematically from a low value soon after birth, rising through early childhood. The early post-birth values can be explained by the influence of .sup.2 H-depleted lipids from mother's breastmilk and the later [delta].sup.2 H rise is consistent with, among other things, a substantial portion of boiled foodstuffs, such as the higher [delta].sup.2 H values observed in porridge. Overall [delta].sup.2 H in dentin shows great promise to elucidate infant and childhood feeding practices, and especially the introduction of supplementary foods during the weaning process.