pH effect on strain-specific transcriptomes of the take-all fungus

The soilborne fungus Gaeumannomyces tritici (G. tritici) causes the take-all disease on wheat roots. Ambient pH has been shown to be critical in different steps of G. tritici life cycle such as survival in bulk soil, saprophytic growth, and pathogenicity on plants. There are however intra-specific v...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-07, Vol.15 (7), p.e0236429-e0236429
Main Authors: Gazengel, Kévin, Lebreton, Lionel, Lapalu, Nicolas, Amselem, Joëlle, Guillerm-Erckelboudt, Anne-Yvonne, Tagu, Denis, Daval, Stéphanie
Format: Article
Language:English
Subjects:
Amino acids
Binding sites
Biology and Life Sciences
Cell proliferation
Environmental Sciences
Fatty acids
Fungi
Gaeumannomyces graminis tritici
Gene expression
Genes
Genomes
Life cycles
Life Sciences
Medicine and Health Sciences
Metabolic pathways
Pathogenesis
Pathogenicity
Pathogens
pH effects
Quality control
Signal transduction
Soils
Take-all disease
Transcription factors
Wheat
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The soilborne fungus Gaeumannomyces tritici (G. tritici) causes the take-all disease on wheat roots. Ambient pH has been shown to be critical in different steps of G. tritici life cycle such as survival in bulk soil, saprophytic growth, and pathogenicity on plants. There are however intra-specific variations and we previously found two types of G. tritici strains that grow preferentially either at acidic pH or at neutral/alkaline pH; gene expression involved in pH-signal transduction pathway and pathogenesis was differentially regulated in two strains representative of these types. To go deeper in the description of the genetic pathways and the understanding of this adaptative mechanism, transcriptome sequencing was achieved on two strains (PG6 and PG38) which displayed opposite growth profiles in two pH conditions (acidic and neutral). PG6, growing better at acidic pH, overexpressed in this condition genes related to cell proliferation. In contrast, PG38, which grew better at neutral pH, overexpressed in this condition genes involved in fatty acids and amino acid metabolisms, and genes potentially related to pathogenesis. This strain also expressed stress resistance mechanisms at both pH, to assert a convenient growth under various ambient pH conditions. These differences in metabolic pathway expression between strains at different pH might buffer the effect of field or soil variation in wheat fields, and explain the success of the pathogen.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0236429