Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer

In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progressi...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-08, Vol.15 (8), p.e0236811-e0236811
Main Authors: Jung, Jiyoon, Jeong, Sanghoon, Jeong, Hoiseon, Oh, Hwa Eun, Choi, Jung-Woo, Lee, Eung Seok, Kim, Young-Sik, Kwak, Yoonjin, Kim, Woo Ho, Lee, Ju-Han
Format: Article
Language:English
Subjects:
Biobanks
Biology and Life Sciences
Biomarkers
Cancer
Development and progression
Diagnosis
Diagnostic software
Diagnostic systems
Gastric cancer
Gene expression
Gene sequencing
Genes
Genetic aspects
Genomes
Health aspects
Hospitals
Hybridization
Identification and classification
Immunohistochemistry
Lymphatic system
Medical prognosis
Medical schools
Medicine
Medicine and Health Sciences
Messenger RNA
Metastasis
Methods
Pathology
Physiological aspects
Prognosis
Prostate cancer
Proteins
Reagents
Real time
Research and analysis methods
Ribonucleic acid
RNA
Software
Stomach cancer
Target recognition
Thermal cycling
Tumor markers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progression of cancer, which was validated by quantitative real time PCR and RNA in-situ hybridization. To compare the protein expression of HOXC6, we evaluated GC and normal gastric tissue samples using western blot analysis and immunohistochemistry. We detected significantly higher levels of HOXC6 in the GC tissues than in the normal controls at both mRNA and protein levels. The expression levels of HOXC6 mRNA in patients with advanced gastric cancer (AGC) were significantly higher than those in patients with early gastric cancer (EGC). Kaplan-Meier curves showed that high expression of HOXC6 mRNA is significantly associated with poor clinical prognosis. Our findings suggest that HOXC6 mRNA may be a novel biomarker and can be potentially valuable in predicting the prognosis of GC patients. Especially, HOXC6 mRNA in-situ hybridization may be a diagnostic tool for predicting prognosis of individual GC patients.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0236811