Translational research into the effects of cigarette smoke on inflammatory mediators and epithelial TRPV1 in Crohn’s disease

• Published in: PloS one 2020-08, Vol.15 (8), p.e0236657-e0236657
• Main Authors: Allais, Liesbeth, Verschuere, Stephanie, Maes, Tania, De Smet, Rebecca, Devriese, Sarah, Gonzales, Gerard Bryan, Peeters, Harald, Van Crombruggen, Koen, Bachert, Claus, De Vos, Martine, Brusselle, Guy G, Bracke, Ken R, Cuvelier, Claude A, Laukens, Debby, Body-Malapel, Mathilde
• Format: Article
• Language: English
• Subjects: Biology and Life Sciences; Biopsy; Capsaicin receptors; Care and treatment; Cigarette smoke; Cigarette smoking; Cigarettes; Colitis; Colon; Crohn's disease; Cytokines; Development and progression; Digestive system; Digestive tract; Disease; Environmental risk; Ethics; Exposure; Forensic pathology; Funding; Gastroenterology; Gastrointestinal system; Gastrointestinal tract; Gene expression; Genetic aspects; Health aspects; Hospitals; Ileum; Immune system; Immunologic factors; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Interleukin 1; Interleukin 8; Intestine; Laboratories; Medical research; Medicin och hälsovetenskap; Medicine; Medicine and Health Sciences; Methods; Modulators; Research and Analysis Methods; Risk analysis; Risk factors; Smoke; Smoking; Social Sciences; Software; Supervision; Transcription; Transient receptor potential proteins; Transport proteins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0236657

Crohn's disease is a pathological condition of the gastro-intestinal tract, causing severe transmural inflammation in the ileum and/or colon. Cigarette smoking is one of the best known environmental risk factors for the development of Crohn's disease. Nevertheless, very little is known about the effect of prolonged cigarette smoke exposure on inflammatory modulators in the gut. We examined the effect of cigarette smoke on cytokine profiles in the healthy and inflamed gut of human subjects and in the trinitrobenzene sulphonic acid mouse model, which mimics distal Crohn-like colitis. In addition, the effect of cigarette smoke on epithelial expression of transient receptor potential channels and their concurrent increase with cigarette smoke-augmented cytokine production was investigated. Active smoking was associated with increased IL-8 transcription in ileum of controls (p < 0,001; n = 18-20/group). In the ileum, TRPV1 mRNA levels were decreased in never smoking Crohn's disease patients compared to healthy subjects (p