Cerebrospinal fluid PCR: A new approach for the diagnosis of CNS sporotrichosis

Kano and colleagues (2003) designed species-specific primers for polymerase chain reaction (PCR) based on Sporothrix schenckii sensu lato chitin synthase 1 (CHS1) gene sequence and applied it in skin biopsy paraffin block [5]. The nested PCR targeting the partial sequence of the 18S rRNA gene was th...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2020-07, Vol.14 (7), p.e0008196-e0008196
Main Authors: Oliveira, Manoel Marques Evangelista, Muniz, Mauro de Medeiros, Almeida-Paes, Rodrigo, Zancope-Oliveira, Rosely Maria, Freitas, Andrea D'Avila, Lima, Marco A, Gutierrez-Galhardo, Maria Clara, Freitas, Dayvison Francis Saraiva
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Biopsy
Care and treatment
Central nervous system diseases
Cerebrospinal fluid
Chitin
Chitin synthase
Deoxyribonucleic acid
Diagnosis
DNA
Fungi
Innovations
Medicine and Health Sciences
Molecular diagnostic techniques
Nervous system
Neurotropism
Nucleotide sequence
Paraffin
Paraffins
Patients
PCR
Polymerase chain reaction
Primers
Protocols
Research and Analysis Methods
rRNA 18S
Sequencing
Skin
Sporotrichosis
Sterilization
Symposium
Testing
Tropical diseases
Vector-borne diseases
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Summary:Kano and colleagues (2003) designed species-specific primers for polymerase chain reaction (PCR) based on Sporothrix schenckii sensu lato chitin synthase 1 (CHS1) gene sequence and applied it in skin biopsy paraffin block [5]. The nested PCR targeting the partial sequence of the 18S rRNA gene was the best choice in terms of sensitivity due to a low fungal burden in CSF [6–7]. Since the beginning of the hyperendemic sporotrichosis in 1998, patients with disseminated sporotrichosis followed up at the Instituto Nacional de Infectologia Evandro Chagas (INI), Fundação Oswaldo Cruz (Fiocruz), undergo a protocol with lumbar puncture because of the possible neurotropism of S. brasiliensis, the main involved species in this region. Discussion CNS sporotrichosis is a challenge and is associated with a worsening of prognosis due to the difficult CSF sterilization [2]. [...]it is pivotal a faster and more effective method for recognizing the fungus dissemination than mycological culture. [...]our clinical sample had the presence of a Sporothrix sensu lato.
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0008196