Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B

To better understand anti-malaria protective immune responses, we examined the cellular mechanisms that govern protective immunity in a murine Plasmodium yoelii 17X NL (PyNL) re-infection model. Initially, we confirmed that immune B cells generated during a primary PyNL infection were largely respon...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-09, Vol.15 (9), p.e0238493-e0238493
Main Authors: Parra, Marcela, Weitner, Megan, Yang, Amy, Akue, Adovi, Liu, Xia, Schmidt, Thomas, Allman, Windy R., Akkoyunlu, Mustafa, Derrick, Steven C.
Format: Article
Language:English
Subjects:
Antibodies
Biological products
Biology and Life Sciences
Biomolecules
CD19 antigen
CD73 antigen
CD80 antigen
Food
Granzyme B
Immunoglobulin M
Immunological memory
Infections
Laboratory animals
Lymphocytes B
Malaria
Medicine and Health Sciences
Memory cells
Parasites
Pathogens
Plasmodium yoelii
Research and Analysis Methods
Spleen
Tropical diseases
Vaccines
Vector-borne diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To better understand anti-malaria protective immune responses, we examined the cellular mechanisms that govern protective immunity in a murine Plasmodium yoelii 17X NL (PyNL) re-infection model. Initially, we confirmed that immune B cells generated during a primary PyNL infection were largely responsible for protection from a second PyNL infection. Using the previously identified memory B cell markers CD80, PD-L2, and CD73, we found an increase in the frequency of CD80-PD-L2-CD73+ B cells up to 55 days after a primary PyNL infection and at 4–6 days following a second PyNL infection. Moreover, injection of enriched immune CD19+CD73+ B cells into nonimmune mice were significantly more protective against a PyNL infection than CD73- B cells. Interestingly, a substantial fraction of these CD73+ B cells also expressed IgM and granzyme B, a biomolecule that has been increasingly associated with protective responses against malaria.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0238493