Differential gene expression elicited by ZIKV infection in trophoblasts from congenital Zika syndrome discordant twins

• Published in: PLoS neglected tropical diseases 2020-08, Vol.14 (8), p.e0008424-e0008424
• Main Authors: Amaral, Murilo Sena, Goulart, Ernesto, Caires-Júnior, Luiz Carlos, Morales-Vicente, David Abraham, Soares-Schanoski, Alessandra, Gomes, Roselane Paiva, Olberg, Giovanna Goncalves de Oliveira, Astray, Renato Mancini, Kalil, Jorge E, Zatz, Mayana, Verjovski-Almeida, Sergio
• Format: Article
• Language: English
• Subjects: Abnormalities; Adhesion; Biology and Life Sciences; Cell activation; Cell adhesion; Cells; Chemokines; Defence mechanisms; Endothelial cells; Extracellular; Extracellular matrix; Fetal diseases; Fetuses; Foetus; Gene expression; Genes; Genetic abnormalities; Genetic aspects; Health aspects; Human influences; Immune response; Immune system; Immunity; Infections; Interferon; Leukocytes; Man-induced effects; Medicine and Health Sciences; Microcephaly; Microencephaly; Mothers; Neural stem cells; Nucleic acids; Perinatal infection; Phenotypes; Placenta; Pluripotency; Pregnancy; Progenitor cells; RANTES; Receptors; Ribonucleic acid; Risk factors; RNA; Stem cells; Supervision; Susceptibility; Trophoblasts; Tropical diseases; Twins; Vector-borne diseases; Viral infections; Viral research; Viruses; Women; Zika virus; Zika virus infection
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0008424

Zika virus (ZIKV) causes congenital Zika syndrome (CZS), which is characterized by fetal demise, microcephaly and other abnormalities. ZIKV in the pregnant woman circulation must cross the placental barrier that includes fetal endothelial cells and trophoblasts, in order to reach the fetus. CZS occurs in ~1-40% of cases of pregnant women infected by ZIKV, suggesting that mothers' infection by ZIKV during pregnancy is not deterministic for CZS phenotype in the fetus. Therefore, other susceptibility factors might be involved, including the host genetic background. We have previously shown that in three pairs of dizygotic twins discordant for CZS, neural progenitor cells (NPCs) from the CZS-affected twins presented differential in vitro ZIKV susceptibility compared with NPCs from the non-affected. Here, we analyzed human-induced-pluripotent-stem-cell-derived (hiPSC-derived) trophoblasts from these twins and compared by RNA-Seq the trophoblasts from CZS-affected and non-affected twins. Following in vitro exposure to a Brazilian ZIKV strain (ZIKV.sup.BR ), trophoblasts from CZS-affected twins were significantly more susceptible to ZIKV.sup.BR infection when compared with trophoblasts from the non-affected. Transcriptome profiling revealed no differences in gene expression levels of ZIKV candidate attachment factors, IFN receptors and IFN in the trophoblasts, either before or after ZIKV.sup.BR infection. Most importantly, ZIKV.sup.BR infection caused, only in the trophoblasts from CZS-affected twins, the downregulation of genes related to extracellular matrix organization and to leukocyte activation, which are important for trophoblast adhesion and immune response activation. In addition, only trophoblasts from non-affected twins secreted significantly increased amounts of chemokines RANTES/CCL5 and IP10 after infection with ZIKV.sup.BR . Overall, our results showed that trophoblasts from non-affected twins have the ability to more efficiently activate genes that are known to play important roles in cell adhesion and in triggering the immune response to ZIKV infection in the placenta, and this may contribute to predict protection from ZIKV dissemination into fetuses' tissues.