Genomic analysis of cardiac surgery-associated Mycobacterium chimaera infections in Italy

One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surg...

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Bibliographic Details
Published in:PloS one 2020-09, Vol.15 (9), p.e0239273
Main Authors: Ghodousi, Arash, Borroni, Emanuele, Peracchi, Marta, Palù, Giorgio, Fallico, Loredana, Rassu, Mario, Manfrin, Vinicio, Mantegani, Paola, Monzillo, Vincenzina, Manganelli, Riccardo, Tortoli, Enrico, Cirillo, Daniela Maria, Ozer, Egon Anderson
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Cardiovascular surgery
Care and treatment
Complications and side effects
Computer and Information Sciences
Development and progression
Engineering and Technology
Epidemics
Gene sequencing
Genetic aspects
Genomes
Genomic analysis
Health aspects
Heart
Heart surgery
Immunology
Infections
Infectious diseases
Medicine and Health Sciences
Mycobacterium chimaera
Mycobacterium infections
Pathogens
Patients
Phylogenetics
Phylogeny
Single nucleotide polymorphisms
Strain analysis
Subgroups
Surgery
Water shortages
Water supply
Whole genome sequencing
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Summary:One hundred and twenty-two Mycobacterium chimaera strains isolated in Italy from cardiac surgery-related patients, cardiac surgery-unrelated patients and from heater-cooler units, were submitted to whole-genome sequencing and to subsequent SNP analysis. All but one strains isolated from cardiac surgery-related patients belonged to Subgroup 1.1 (19/23) or Subgroup 1.8 (3/23). Only 28 out of 79 strains isolated from heater-cooler units belonged to groupings other than 1.1 and 1.8. The strains isolated from cardiac surgery-unrelated patients were instead distributed across the phylogenetic tree. Our data, the first on isolates from Italy, are in agreement with a recent large genomic study suggesting a common source, represented by strains belonging to Subgroups 1.1 and 1.8, of cardiac surgery-related Mycobacterium chimaera infections. The strains belonging to groupings other than 1.1 and 1.8 isolated from heather-cooler units evidently resulted from contaminations at hospital level and had no share in the Mycobacterium chimaera outbreak. One Mycobacterium chimaera strain investigated in this study proved distant from every previously known Mycobacterium chimaera Groups (1, 2, 3 and 4) and we propose to assign to a novel group, named "Group 5".
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0239273