Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats

• Published in: PloS one 2020-09, Vol.15 (9), p.e0239660-e0239660
• Main Authors: Matsui, Saori, Yoneda, Naomichi, Maezono, Hazuki, Kuremoto, Katsutaka, Ishimoto, Takuya, Nakano, Takayoshi, Yumoto, Hiromichi, Ebisu, Shigeyuki, Noiri, Yuichiro, Hayashi, Mikako
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Bacteria; Bacteria - genetics; Bacteria - isolation & purification; Bacterial infections; Biology and Life Sciences; Bone surgery; Care and treatment; Composite materials; Dental Pulp Cavity - diagnostic imaging; Dental Pulp Cavity - pathology; Dental roots; Dentistry; Diathermy; DNA, Bacterial - isolation & purification; DNA, Bacterial - metabolism; Electrodes; Endodontics; Engineering and Technology; Engineering schools; Fibroblast growth factor 2; Fibroblast Growth Factor 2 - metabolism; Gene expression; Graduate studies; Growth factors; Gum disease; Imaging, Three-Dimensional; Immune system; Infections; Interleukin-1beta - metabolism; Irradiation; Lesions; Male; Manufacturing; Medicine and Health Sciences; Methods; Molar - pathology; Molars; Periapical Periodontitis - microbiology; Periapical Periodontitis - pathology; Periapical Periodontitis - therapy; Periodontitis; Radiation; Radio Waves; Rats; Rats, Wistar; Root canal therapy; Root Canal Therapy - methods; Root canals; Teeth; Transforming Growth Factor beta1 - metabolism; University graduates; Waves; X-Ray Microtomography
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0239660

The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.