Blood-brain barrier permeability analysis of plant ceramides

Ceramides, a type of sphingolipid, are cell membrane components and lipid mediators that modulate a variety of cell functions. In plants, ceramides are mostly present in a glucosylated glucosylceramide (GlcCer) form. We previously showed that oral administration of konjac-derived GlcCer to a mouse m...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-11, Vol.15 (11), p.e0241640-e0241640
Main Authors: Eguchi, Koichi, Mikami, Daisuke, Sun, Hui, Tsumita, Takuya, Takahashi, Kaori, Mukai, Katsuyuki, Yuyama, Kohei, Igarashi, Yasuyuki
Format: Article
Language:English
Subjects:
Alzheimer's disease
Animal cognition
Animal models
Animals
Biology and Life Sciences
Blood-brain barrier
Blood-Brain Barrier - drug effects
Blood-Brain Barrier - metabolism
Brain
Brain research
Capillary Permeability
Cell culture
Cell membranes
Cells, Cultured
Ceramides
Ceramides - pharmacology
Chromatography
Diet
Ethanol
Ethanolamines - pharmacology
Experiments
Fatty acids
Health aspects
Life sciences
Lipids
Medicine and Health Sciences
Membrane permeability
Metabolites
Mice
Mice, Inbred BALB C
Neurodegenerative diseases
Oral administration
Permeability
Physical Sciences
Plant products
Research and Analysis Methods
Senile plaques
Signal transduction
Sphingomyelin
Sphingomyelins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ceramides, a type of sphingolipid, are cell membrane components and lipid mediators that modulate a variety of cell functions. In plants, ceramides are mostly present in a glucosylated glucosylceramide (GlcCer) form. We previously showed that oral administration of konjac-derived GlcCer to a mouse model of Alzheimer's disease reduced brain amyloid-β and amyloid plaques. Dietary plant GlcCer compounds are absorbed as ceramides, but it is unclear whether they can cross the blood-brain barrier (BBB). Herein, we evaluated the BBB permeability of synthetic plant-type ceramides (4, 8-sphingadienine, d18:2) using mouse and BBB cell culture models, and found that they could permeate the BBB both in vivo and in vitro. In addition, administrated ceramides were partially metabolized to other sphingolipid species, namely sphingomyelin (SM) and GlcCer, while crossing the BBB. Thus, plant ceramides can cross the BBB, suggesting that ceramides and their metabolites might affect brain functions.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0241640