Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)

The extensive use of nanomaterials generates toxic effects on non-target species and the ecosystem. Although gold nanoparticles (Au-NPs) are generally expected to be safe, the recent study contains conflicting data regarding their cytotoxicity in the darkling beetles Trachyderma hispida. The study p...

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Published in:PloS one 2020-11, Vol.15 (11), p.e0241837
Main Authors: El-Ashram, Saeed, Kheirallah, Dalia Abdel Moneim, El-Samad, Lamia Moustafa, Toto, Noura A
Format: Article
Language:English
Subjects:
Animals
Annexin V
Anomalies
Antioxidants
Apoptosis
Beetles
Biology and Life Sciences
Cell growth
Cell Survival - drug effects
Coleoptera
Cytotoxicity
DNA nucleotidylexotransferase
Engineering and Technology
Environmental changes
Enzymes
Fecundity
Female
Females
Flow cytometry
Gene expression
Gene Expression Profiling
Gene Expression Regulation - drug effects
Genotoxicity
Glutathione
Glutathione Peroxidase - genetics
Gold
Gold - adverse effects
Insects
Life sciences
Medicine and Health Sciences
Metal Nanoparticles
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Nanomaterials
Nanoparticles
Nanotechnology
Observations
Ovaries
Ovary - cytology
Ovary - drug effects
Ovary - metabolism
Oxidative stress
Perturbation
Physiological aspects
Selenium
Sublethal dosage
Tenebrio - drug effects
Tenebrio - genetics
Tenebrionidae
Toxicity
Trachyderma hispida
Transcription
Ultrastructure
Zoology
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cited_by cdi_FETCH-LOGICAL-c692t-313a90194ace4b1c16e29afcc2bc4df8d186daf0d9bb35be83d4caca7c255cd3
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creator El-Ashram, Saeed
Kheirallah, Dalia Abdel Moneim
El-Samad, Lamia Moustafa
Toto, Noura A
description The extensive use of nanomaterials generates toxic effects on non-target species and the ecosystem. Although gold nanoparticles (Au-NPs) are generally expected to be safe, the recent study contains conflicting data regarding their cytotoxicity in the darkling beetles Trachyderma hispida. The study postulated cellular perturbation in the ovarian tissue of the beetles induced by a sublethal dose of Au-NPs (0.01 mg/g). When compared with the controls, a significant inhibition in the activities of the antioxidant enzymes selenium-dependent (GPOX) and selenium-independent (GSTP) glutathione peroxidases (GPx) was observed in the treated beetles. The study proposed microRNAs (miRNA-282 and miRNA-989) as genotoxic markers for the first time, reporting a significant suppression in their transcriptional levels in the treated beetles. Furthermore, TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and flow cytometry assays (annexin V-Fitc) indicated a significant increase in ovarian cell apoptosis in the treated beetles. Additionally, an ultrastructure examination revealed pathological changes in the ovarian cells of the treated beetles. The resulting anomalies in the present study may interrupt the fecundity of the beetles and lead to the future suppression of beetle populations.
doi_str_mv 10.1371/journal.pone.0241837
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source Open Access: PubMed Central; Publicly Available Content Database
subjects Animals
Annexin V
Anomalies
Antioxidants
Apoptosis
Beetles
Biology and Life Sciences
Cell growth
Cell Survival - drug effects
Coleoptera
Cytotoxicity
DNA nucleotidylexotransferase
Engineering and Technology
Environmental changes
Enzymes
Fecundity
Female
Females
Flow cytometry
Gene expression
Gene Expression Profiling
Gene Expression Regulation - drug effects
Genotoxicity
Glutathione
Glutathione Peroxidase - genetics
Gold
Gold - adverse effects
Insects
Life sciences
Medicine and Health Sciences
Metal Nanoparticles
MicroRNA
MicroRNAs
MicroRNAs - genetics
miRNA
Nanomaterials
Nanoparticles
Nanotechnology
Observations
Ovaries
Ovary - cytology
Ovary - drug effects
Ovary - metabolism
Oxidative stress
Perturbation
Physiological aspects
Selenium
Sublethal dosage
Tenebrio - drug effects
Tenebrio - genetics
Tenebrionidae
Toxicity
Trachyderma hispida
Transcription
Ultrastructure
Zoology
title Relative expression of microRNAs, apoptosis, and ultrastructure anomalies induced by gold nanoparticles in Trachyderma hispida (Coleoptera: Tenebrionidae)
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