Hsp104-dependent ability to assimilate mannitol and sorbitol conferred by a truncated Cyc8 with a C-terminal polyglutamine in Saccharomyces cerevisiae

Tup1-Cyc8 (also known as Tup1-Ssn6) is a general transcriptional corepressor. D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but acquires the ability to assimilate mannitol due to a...

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Published in:PloS one 2020-11, Vol.15 (11), p.e0242054-e0242054
Main Authors: Tanaka, Hideki, Murata, Kousaku, Hashimoto, Wataru, Kawai, Shigeyuki
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Assimilation
Binding sites
Biology and Life Sciences
Biotechnology
Carbon
Codon, Nonsense
D-Sorbitol
Dehydrogenases
Deoxyribonucleic acid
DNA
Food science
Gene Expression Regulation, Fungal
Genotype & phenotype
Glucose
Glutamine
Glycerol
Guanidine hydrochloride
Heat-Shock Proteins - metabolism
Influence
Laboratories
Mannitol
Mannitol - metabolism
Mutation
Nuclear Proteins - genetics
Peptides - metabolism
Physical Sciences
Physiological aspects
Plasmids
Polyglutamine
Polyols
Promoter Regions, Genetic
Protein Domains
Proteins
Repressor Proteins - chemistry
Repressor Proteins - genetics
Repressor Proteins - metabolism
Research and Analysis Methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sorbitol
Sorbitol - metabolism
Transcription
Trinucleotide repeat diseases
Yeast
Yeasts
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creator Tanaka, Hideki
Murata, Kousaku
Hashimoto, Wataru
Kawai, Shigeyuki
description Tup1-Cyc8 (also known as Tup1-Ssn6) is a general transcriptional corepressor. D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but acquires the ability to assimilate mannitol due to a spontaneous mutation in TUP1 or CYC8. In this study, we found that spontaneous mutation of TUP1 or CYC8 also permitted assimilation of sorbitol. Some spontaneous nonsense mutations of CYC8 produced a truncated Cyc8 with a C-terminal polyglutamine. The effects were guanidine hydrochloride-sensitive and were dependent on Hsp104, but were complemented by introduction of CYC8, ruling out involvement of a prion. Assimilation of mannitol and sorbitol conferred by other mutations of TUP1 or CYC8 was guanidine hydrochloride-tolerant. It is physiologically reasonable that S. cerevisiae carries this mechanism to acquire the ability to assimilate major polyols in nature.
doi_str_mv 10.1371/journal.pone.0242054
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D-Mannitol (mannitol) and D-sorbitol (sorbitol) are the major polyols in nature. Budding yeast Saccharomyces cerevisiae is unable to assimilate mannitol or sorbitol, but acquires the ability to assimilate mannitol due to a spontaneous mutation in TUP1 or CYC8. In this study, we found that spontaneous mutation of TUP1 or CYC8 also permitted assimilation of sorbitol. Some spontaneous nonsense mutations of CYC8 produced a truncated Cyc8 with a C-terminal polyglutamine. The effects were guanidine hydrochloride-sensitive and were dependent on Hsp104, but were complemented by introduction of CYC8, ruling out involvement of a prion. Assimilation of mannitol and sorbitol conferred by other mutations of TUP1 or CYC8 was guanidine hydrochloride-tolerant. It is physiologically reasonable that S. cerevisiae carries this mechanism to acquire the ability to assimilate major polyols in nature.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33175887</pmid><doi>10.1371/journal.pone.0242054</doi><tpages>e0242054</tpages><orcidid>https://orcid.org/0000-0002-3640-9540</orcidid><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
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1932-6203
language eng
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source Publicly Available Content Database; PubMed Central
subjects Amino acids
Analysis
Assimilation
Binding sites
Biology and Life Sciences
Biotechnology
Carbon
Codon, Nonsense
D-Sorbitol
Dehydrogenases
Deoxyribonucleic acid
DNA
Food science
Gene Expression Regulation, Fungal
Genotype & phenotype
Glucose
Glutamine
Glycerol
Guanidine hydrochloride
Heat-Shock Proteins - metabolism
Influence
Laboratories
Mannitol
Mannitol - metabolism
Mutation
Nuclear Proteins - genetics
Peptides - metabolism
Physical Sciences
Physiological aspects
Plasmids
Polyglutamine
Polyols
Promoter Regions, Genetic
Protein Domains
Proteins
Repressor Proteins - chemistry
Repressor Proteins - genetics
Repressor Proteins - metabolism
Research and Analysis Methods
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - chemistry
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Sorbitol
Sorbitol - metabolism
Transcription
Trinucleotide repeat diseases
Yeast
Yeasts
title Hsp104-dependent ability to assimilate mannitol and sorbitol conferred by a truncated Cyc8 with a C-terminal polyglutamine in Saccharomyces cerevisiae
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