Adding MASP1 to the lectin pathway-Leprosy association puzzle: Hints from gene polymorphisms and protein levels

Deposition of complement factors on Mycobacterium leprae may enhance phagocytosis. Such deposition may occur through the lectin pathway of complement. Three proteins of the lectin pathway are produced from the gene MASP1: Mannan-binding lectin-associated serine protease 1 (MASP-1) and MASP-3 and man...

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Bibliographic Details
Published in:PLoS neglected tropical diseases 2020-04, Vol.14 (4), p.e0007534-e0007534
Main Authors: Weinschutz Mendes, Hellen, Boldt, Angelica Beate Winter, von Rosen Seeling Stahlke, Ewalda, Jensenius, Jens Christian, Thiel, Steffen, Messias-Reason, Iara J Taborda
Format: Article
Language:English
Subjects:
Binding
Binding sites
Biology and Life Sciences
Blood & organ donations
Cellular signal transduction
CpG islands
Deposition
Development and progression
DNA
DNA methylation
Funding
Gene polymorphism
Genetic aspects
Genetic polymorphisms
Haplotypes
Hospitals
Kinases
Laboratories
Lectins
Leprosy
Levels
Macrophages
Mannan
Mannose-binding lectin
MASP-1 protein
Medicine and Health Sciences
Microbiological research
mRNA
Mycobacterium leprae
Nucleotide sequence
Pathogens
Pathology
Patients
PCR
Phagocytosis
Proteases
Proteins
Research and Analysis Methods
Serine
Serine proteinase
Serum
Serum levels
Supervision
Tropical diseases
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Summary:Deposition of complement factors on Mycobacterium leprae may enhance phagocytosis. Such deposition may occur through the lectin pathway of complement. Three proteins of the lectin pathway are produced from the gene MASP1: Mannan-binding lectin-associated serine protease 1 (MASP-1) and MASP-3 and mannan-binding lectin-associated protein of 44 kDa (MAp44). Despite their obvious importance, the roles played by these proteins have never been investigated in leprosy disease. We haplotyped five MASP1 polymorphisms by multiplex sequence-specific PCR (intronic rs7609662*G>A and rs13064994*C>T, exon 12 3'-untranslated rs72549262*C>G, rs1109452*C>T and rs850314*G>A) and measured MASP-1, MASP-3 and MAp44 serum levels in 196 leprosy patients (60%, lepromatous) and 193 controls. Lower MASP-3 and MAp44 levels were observed in patients, compared with controls (P = 0.0002 and P
ISSN:1935-2735
1935-2727
1935-2735
DOI:10.1371/journal.pntd.0007534