MiR-424-5p regulates cell cycle and inhibits proliferation of hepatocellular carcinoma cells by targeting E2F7

• Published in: PloS one 2020-11, Vol.15 (11), p.e0242179-e0242179
• Main Authors: Zhao, Yichao, Zhu, Chaoqian, Chang, Qing, Peng, Peng, Yang, Jie, Liu, Chunmei, Liu, Yang, Chen, Xiaonan, Liu, Yuanguang, Cheng, Ran, Wu, Yijie, Wu, Xiaotang, Hu, Liang, Yin, Jun
• Format: Article
• Language: English
• Subjects: Bioinformatics; Biology and Life Sciences; Cancer cells; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Care and treatment; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cholecystokinin; Composition; Development and progression; Down-Regulation; E2F7 Transcription Factor - genetics; E2F7 Transcription Factor - metabolism; Ectopic expression; Engineering research; Flow cytometry; G1 phase; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; HEK293 Cells; Hepatocellular carcinoma; Humans; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Medical prognosis; Medicine and Health Sciences; Metastasis; MicroRNA; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; mRNA; Proteins; Research and Analysis Methods; S phase; Surgery; Target recognition; Transcription factors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0242179

This study aims to explore the mechanism of the miR-424-5p/E2F7 axis in hepatocellular carcinoma (HCC) and provide new ideas for targeted therapy of HCC. Bioinformatics analysis was used to identify the target differentially expressed miRNA in HCC and predict its target gene. qRT-PCR was employed to verify the expression of miR-424-5p and E2F7 mRNA in HCC cells. Western blot was performed to detect the effect of miR-424-5p ectopic expression on the protein expression of E2F7. CCK-8 was used to detect proliferative activity of HCC cells and flow cytometry was carried out for analyzing cell cycle distribution. Dual luciferase reporter assay was conducted to verify the direct targeting relationship between miR-424-5p and E2F7. We observed that miR-424-5p was down-regulated in HCC cells. CCK-8 showed that overexpression of miR-424-5p inhibited cell proliferation, and flow cytometry showed that miR-424-5p could block cells in G0/G1 phase. E2F7 was up-regulated in HCC cells, and E2F7 overexpression could facilitate the proliferative ability of HCC cells and promote the cell cycle progressing from G0/G1 to S phase. Furthermore, dual-luciferase reporter assay indicated that miR-424-5p could directly down-regulate E2F7 expression. Analysis on cell function demonstrated that miR-424-5p inhibited the proliferation of HCC cells and blocked cell cycle at G0/G1 phase by targeting E2F7. Our results proved that E2F7 was a direct target of miR-424-5p, and miR-424-5p could regulate cell cycle and further inhibit the proliferation of HCC cells by targeting E2F7.