Interaction of preimplantation factor with the global bovine endometrial transcriptome

Preimplantation factor (PIF) is an embryo derived peptide which exerts an immune modulatory effect on human endometrium, promoting immune tolerance to the embryo whilst maintaining the immune response to invading pathogens. While bovine embryos secrete PIF, the effect on the bovine endometrium is un...

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Bibliographic Details
Published in:PloS one 2020-12, Vol.15 (12), p.e0242874
Main Authors: Wonfor, Ruth E, Creevey, Christopher J, Natoli, Manuela, Hegarty, Matthew, Nash, Deborah M, Rose, Michael T
Format: Article
Language:English
Subjects:
Animals
Biology and Life Sciences
Biopsy
Biosynthesis
Cattle
Crosstalk
Embryos
Endometrium
Endometrium - drug effects
Endometrium - metabolism
Explants
Female
Fertility
Gene expression
Gene Ontology
Gene regulation
Gene sequencing
Genes
Genetic aspects
Immune response
Immune system
Immunological tolerance
Interleukin 17
MAP kinase
Medical research
Medicine and Health Sciences
Morphology
NF-κB protein
Ovaries
Pathogens
Peptides
Peptides - pharmacology
Physiological aspects
Pregnancy
Progesterone - metabolism
Proteins
Ribonucleic acid
RNA
Signal transduction
Steroids
Time Factors
Tissue culture
Transcription factors
Transcriptome - drug effects
Tumor necrosis factor
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Summary:Preimplantation factor (PIF) is an embryo derived peptide which exerts an immune modulatory effect on human endometrium, promoting immune tolerance to the embryo whilst maintaining the immune response to invading pathogens. While bovine embryos secrete PIF, the effect on the bovine endometrium is unknown. Maternal recognition of pregnancy is driven by an embryo-maternal cross talk, however the process differs between humans and cattle. As many embryos are lost during the early part of pregnancy in cattle, a greater knowledge of factors affecting the embryo-maternal crosstalk, such as PIF, is needed to improve fertility. Therefore, for the first time, we demonstrate the effect of synthetic PIF (sPIF) on the bovine transcriptome in an ex vivo bovine endometrial tissue culture model. Explants were cultured for 30h with sPIF (100nM) or in control media. Total RNA was analysed via RNA-sequencing. As a result of sPIF treatment, 102 genes were differentially expressed compared to the control (Padj
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0242874