Connecting moss lipid droplets to patchoulol biosynthesis

Plant-derived terpenoids are extensively used in perfume, food, cosmetic and pharmaceutical industries, and several attempts are being made to produce terpenes in heterologous hosts. Native hosts have evolved to accumulate large quantities of terpenes in specialized cells. However, heterologous cell...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2020-12, Vol.15 (12), p.e0243620-e0243620
Main Authors: Peramuna, Anantha, Bae, Hansol, Quiñonero López, Carmen, Fromberg, Arvid, Petersen, Bent, Simonsen, Henrik Toft
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Biosynthesis
Biosynthetic Pathways
Biotechnology
Bryopsida - enzymology
Bryopsida - metabolism
Chemical properties
Chemical research
Computer and Information Sciences
Deoxyribonucleic acid
DNA
Droplets
Ectopic expression
Engineering and Technology
Evaporation
Flowers & plants
Food industry
Isomerases - metabolism
Life Sciences
Lipid Droplets - metabolism
Lipids
Mosses
Mutants
Native species
Oleosin
Patchouli oil
Patchoulol
Pharmaceutical industry
Physical Sciences
Physiological aspects
Plant lipids
Plant Proteins - metabolism
Plants
Production processes
Proteins
Research and Analysis Methods
Sesquiterpenes - metabolism
Terpenes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Plant-derived terpenoids are extensively used in perfume, food, cosmetic and pharmaceutical industries, and several attempts are being made to produce terpenes in heterologous hosts. Native hosts have evolved to accumulate large quantities of terpenes in specialized cells. However, heterologous cells lack the capacity needed to produce and store high amounts of non-native terpenes, leading to reduced growth and loss of volatile terpenes by evaporation. Here, we describe how to direct the sesquiterpene patchoulol production into cytoplasmic lipid droplets (LDs) in Physcomitrium patens (syn. Physcomitrella patens), by attaching patchoulol synthase (PTS) to proteins linked to plant LD biogenesis. Three different LD-proteins: Oleosin (PpOLE1), Lipid Droplet Associated Protein (AtLDAP1) and Seipin (PpSeipin325) were tested as anchors. Ectopic expression of PTS increased the number and size of LDs, implying an unknown mechanism between heterologous terpene production and LD biogenesis. The expression of PTS physically linked to Seipin increased the LD size and the retention of patchoulol in the cell. Overall, the expression of PTS was lower in the anchored mutants than in the control, but when normalized to the expression the production of patchoulol was higher in the seipin-linked mutants.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0243620