An integrative approach reveals five new species of highland papayas (Caricaceae, Vasconcellea) from northern Peru

The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (...

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Bibliographic Details
Published in:PloS one 2020-12, Vol.15 (12), p.e0242469-e0242469
Main Authors: Tineo, Daniel, Bustamante, Danilo E, Calderon, Martha S, Mendoza, Jani E, Huaman, Eyner, Oliva, Manuel
Format: Article
Language:English
Subjects:
Agronomy
Biology and Life Sciences
Biomarkers
Carica - classification
Carica - genetics
Caricaceae
Cell Nucleus - genetics
Chloroplasts
Chloroplasts - genetics
Coalescence
Coalescing
Computer and Information Sciences
Deoxyribonucleic acid
Discovery and exploration
DNA
DNA, Chloroplast - genetics
DNA, Intergenic - genetics
Genes, Plant
Genetic distance
Genetic markers
Identification and classification
Methods
Morphology
Multilocus Sequence Typing
New species
Papaya
Papayas
Peru
Phylogenetics
Phylogeny
Physiological aspects
Sequence Analysis, DNA
Species diversity
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Summary:The assignment of accurate species names is crucial, especially for those with confirmed agronomic potential such as highland papayas. The use of additional methodologies and data sets is recommended to establish well-supported boundaries among species of Vasconcellea. Accordingly, six chloroplast (trnL-trnF, rpl20-rps12, psbA-trnH intergenic spacers, matK and rbcL genes) and nuclear (ITS) markers were used to delimit species in the genus Vasconcellea using phylogeny and four DNA-based methods. Our results demonstrated congruence among different methodologies applied in this integrative study (i.e., morphology, multilocus phylogeny, genetic distance, coalescence methods). Genetic distance (ABGD, SPN), a coalescence method (BPP), and the multilocus phylogeny supported 22-25 different species of Vasconcellea, including the following five new species from northern Peru: V. badilloi sp. nov., V. carvalhoae sp. nov., V. chachapoyensis sp. nov., V. pentalobis sp. nov., and V. peruviensis sp. nov. Genetic markers that gave better resolution for distinguishing species were ITS and trnL-trnF. Phylogenetic diversity and DNA-species delimitation methods could be used to discover taxa within traditionally defined species.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0242469