Systematic identification of autophagy-related proteins in Aedes albopictus

Autophagy is a conserved cellular process playing a role in maintenance of cellular homeostasis and response to changing nutrient conditions via degradation and recirculation of cellular redundant components. Autophagy-related proteins (Atg) play important function in autophagy pathway. Aedes albopi...

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Published in:PloS one 2021-01, Vol.16 (1), p.e0245694-e0245694
Main Authors: Wang, Yu, Qiao, Jialu, Zhang, Dandan, Zhong, Chunyan, Wang, Shengya, Li, Xiaomei, Feng, Lingyan, Shi, Shen, Wang, Bingxue, Liu, Qingzhen
Format: Article
Language:English
Subjects:
Aedes
Aedes albopictus
Antibodies
Apoptosis
Aquatic insects
Autophagy
Autophagy (Cytology)
Biology and Life Sciences
Cell death
Cloning
Cytoplasm
Dengue
Dengue fever
Disease transmission
Gene expression
Genes
Genetic aspects
Health aspects
Health risks
Homogenization
Identification and classification
Infections
Laboratories
Life sciences
Medicine and Health Sciences
Mosquitoes
Phagocytosis
Physiological aspects
Proteins
Public health
Reagents
Research and Analysis Methods
Vector-borne diseases
Viral infections
Virology
Viruses
Zika virus
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cited_by cdi_FETCH-LOGICAL-c692t-ec9009253b8481c54255c0f69f63b12002bedc0df315c08d7c68fb1e459294403
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container_title PloS one
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creator Wang, Yu
Qiao, Jialu
Zhang, Dandan
Zhong, Chunyan
Wang, Shengya
Li, Xiaomei
Feng, Lingyan
Shi, Shen
Wang, Bingxue
Liu, Qingzhen
description Autophagy is a conserved cellular process playing a role in maintenance of cellular homeostasis and response to changing nutrient conditions via degradation and recirculation of cellular redundant components. Autophagy-related proteins (Atg) play important function in autophagy pathway. Aedes albopictus mosquito is an effective vector transmitting multiple viruses which cause serious human diseases. Moreover, Aedes albopictus mosquito is becoming a serious threat to human health due to its widening distribution in recent years and thus worth of more research attention. It was reported that autophagy might play a role in viral infection in Aedes mosquito. To better understand the interaction between autophagy and arbovirus infection in mosquito system, it is necessary to identify autophagy pathway in the system. However, autophagy in Aedes albopictus mosquito is still poorly understood so far. We recently identified AaAtg8, the first Atg protein reported in Aedes albopictus mosquito. This work further identified twelve atg genes in Aedes albopictus mosquito. Sequence and phylogenetic analysis of the twelve atg genes were performed. Expression profiles of all the twelve Aaatg genes in different developmental stages and genders of Aedes albopictus mosquito were conducted. Effects of chemicals inhibiting or inducing autophagy on the levels of eight identified AaAtg proteins were examined. The function of two identified AaAtg proteins AaAtg6 and AaAtg16 and their response to arbovirus SINV infection were studied preliminarily. Taken together, this work systematically identified Aedes albopictus atg genes and provided basic information which might help to elucidate the autophagy pathway and the role of autophagy in arbovirus infection in Aedes mosquito system.
doi_str_mv 10.1371/journal.pone.0245694
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Expression profiles of all the twelve Aaatg genes in different developmental stages and genders of Aedes albopictus mosquito were conducted. Effects of chemicals inhibiting or inducing autophagy on the levels of eight identified AaAtg proteins were examined. The function of two identified AaAtg proteins AaAtg6 and AaAtg16 and their response to arbovirus SINV infection were studied preliminarily. Taken together, this work systematically identified Aedes albopictus atg genes and provided basic information which might help to elucidate the autophagy pathway and the role of autophagy in arbovirus infection in Aedes mosquito system.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33465164</pmid><doi>10.1371/journal.pone.0245694</doi><tpages>e0245694</tpages><orcidid>https://orcid.org/0000-0003-0426-1921</orcidid><orcidid>https://orcid.org/0000-0003-2955-2768</orcidid><oa>free_for_read</oa></addata></record>
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1932-6203
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source Publicly Available Content Database; PubMed Central
subjects Aedes
Aedes albopictus
Antibodies
Apoptosis
Aquatic insects
Autophagy
Autophagy (Cytology)
Biology and Life Sciences
Cell death
Cloning
Cytoplasm
Dengue
Dengue fever
Disease transmission
Gene expression
Genes
Genetic aspects
Health aspects
Health risks
Homogenization
Identification and classification
Infections
Laboratories
Life sciences
Medicine and Health Sciences
Mosquitoes
Phagocytosis
Physiological aspects
Proteins
Public health
Reagents
Research and Analysis Methods
Vector-borne diseases
Viral infections
Virology
Viruses
Zika virus
title Systematic identification of autophagy-related proteins in Aedes albopictus
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