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A workflow for sizing oligomeric biomolecules based on cryo single molecule localization microscopy

Single molecule localization microscopy (SMLM) has enormous potential for resolving subcellular structures below the diffraction limit of light microscopy: Localization precision in the low digit nanometer regime has been shown to be achievable. In order to record localization microscopy data, howev...

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Bibliographic Details
Published in:PloS one 2021-01, Vol.16 (1), p.e0245693-e0245693
Main Authors: Schneider, Magdalena C, Telschow, Roger, Mercier, Gwenael, López-Martinez, Montserrat, Scherzer, Otmar, Schütz, Gerhard J
Format: Article
Language:English
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Summary:Single molecule localization microscopy (SMLM) has enormous potential for resolving subcellular structures below the diffraction limit of light microscopy: Localization precision in the low digit nanometer regime has been shown to be achievable. In order to record localization microscopy data, however, sample fixation is inevitable to prevent molecular motion during the rather long recording times of minutes up to hours. Eventually, it turns out that preservation of the sample's ultrastructure during fixation becomes the limiting factor. We propose here a workflow for data analysis, which is based on SMLM performed at cryogenic temperatures. Since molecular dipoles of the fluorophores are fixed at low temperatures, such an approach offers the possibility to use the orientation of the dipole as an additional information for image analysis. In particular, assignment of localizations to individual dye molecules becomes possible with high reliability. We quantitatively characterized the new approach based on the analysis of simulated oligomeric structures. Side lengths can be determined with a relative error of less than 1% for tetramers with a nominal side length of 5 nm, even if the assumed localization precision for single molecules is more than 2 nm.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0245693