Could LASCA-imaging of GB-speckles be applied for a high discrimination and typing of pathogenic bacteria?

In this article, the method of analysis of GB-speckles (gene-based speckles) has been adapted to the problem of detecting the differences in a group of genes (usually 5-7), used in Multi Locus Sequence Typing (MLST). This method is based on s-LASCA imaging (spatial Laser Speckle Contrast Analysis) o...

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Bibliographic Details
Published in:PloS one 2021-01, Vol.16 (1), p.e0245657-e0245657
Main Authors: Ulianova, Onega, Ulyanov, Sergey, Zaytsev, Sergey, Saltykov, Yuriy, Ulyanov, Alexander, Feodorova, Valentina
Format: Article
Language:English
Subjects:
Analysis
Auroral kilometric radiation
Bacteria
Bacteria, Pathogenic
Bioinformatics
Biology and Life Sciences
Chlamydia
Computer programs
Diagnostic imaging
Drafting software
Engineering and Technology
Epidemiology
Genes
Genetic aspects
Health aspects
Health physics
Identification and classification
Infectious diseases
Interferometry
Laser radiation
Laser speckle
Lasers
Medical research
Medicine and Health Sciences
Methods
Microbiology
Microorganisms
Molecular evolution
Mutation
Nucleotides
Pandemics
Physics
Polymorphism
Radiation
Research and Analysis Methods
Research facilities
Single nucleotide polymorphisms
Software
Typing
Virology
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Summary:In this article, the method of analysis of GB-speckles (gene-based speckles) has been adapted to the problem of detecting the differences in a group of genes (usually 5-7), used in Multi Locus Sequence Typing (MLST). This method is based on s-LASCA imaging (spatial Laser Speckle Contrast Analysis) of virtual GB-speckle and on the technique of RGB coordinates for GB-speckles, processed by the s-LASCA method. A very high sensitivity and accuracy of the new method for detecting gene polymorphism as a great alternative to classical MLST has been demonstrated. The analysis of GB-speckles, obtained for the concatenated sequences of seven genes (gatA, gidA, enoA, fumC, hemN, hflX, oppA) of three different Chlamydia trachomatis strains (E/Bour, ST94; G/9301, ST95; G/11222, ST94) has been applied as the model. The high efficiency of usage of s-LASCA-imaging of GB-speckles has been shown. The data obtained represent a significant progress in digital biology as a whole and improvements in the bio-digitalization of bacterial DNA.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0245657