Transcriptomic analyses show that 24-epibrassinolide (EBR) promotes cold tolerance in cotton seedlings

In plants, brassinosteroids (BRs) are a class of steroidal hormones that are involved in numerous physiological responses. However, the function of BRs in cold tolerance in cotton has not been explored. In this study, cotton seedlings were treated with five concentrations (0, 0.05, 0.1, 0.2, 0.5 and...

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Bibliographic Details
Published in:PloS one 2021-02, Vol.16 (2), p.e0245070-e0245070
Main Authors: Dou, Lingling, Sun, Yaru, Li, Shuye, Ge, Changwei, Shen, Qian, Li, Huaizhu, Wang, Wenbo, Mao, Jiayi, Xiao, Guanghui, Pang, Chaoyou
Format: Article
Language:English
Subjects:
Abscisic acid
Acclimatization (Plants)
Agricultural research
Agricultural sciences
Biology
Biology and Life Sciences
Biosynthesis
Botanical research
Brassinosteroids
Chemical engineering
Chemistry
Cold
Cold tolerance
Cold treatment
Cotton
Data processing
Drafting software
Electrolyte leakage
Elongation
Funding
Gene expression
Genetic aspects
Glutathione
Gossypol
Hormones
Humidity
Information processing
Laboratories
Life sciences
Malondialdehyde
Metabolism
Photomorphogenesis
Photosynthesis
Physical Sciences
Physiological responses
Physiology
Plant growth
Pollen
Proline
Properties
Seedlings
Seeds
Senescence
Signal transduction
Software
Steroid hormones
Textile fibers
Xylem
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Summary:In plants, brassinosteroids (BRs) are a class of steroidal hormones that are involved in numerous physiological responses. However, the function of BRs in cold tolerance in cotton has not been explored. In this study, cotton seedlings were treated with five concentrations (0, 0.05, 0.1, 0.2, 0.5 and 1.0 mg/L) of 24-Epibrassinolide (EBR) at 4°C. We measured the electrolyte leakage, malondialdehyde (MDA) content, proline content, and net photosynthesis rate (Pn) of the seedlings, which showed that EBR treatment increased cold tolerance in cotton in a dose-dependent manner, and that 0.2 mg/L is an optimum concentration for enhancing cold tolerance. The function of EBR in cotton cotyledons was investigated in the control 0 mg/L (Cold+water) and 0.2 mg/L (Cold+EBR) treatments using RNA-Seq. A total of 4,001 differentially expressed genes (DEGs), including 2,591 up-regulated genes and 1,409 down-regulated genes were identified. Gene Ontology (GO) and biochemical pathway enrichment analyses showed that EBR is involved in the genetic information process, secondary metabolism, and also inhibits abscisic acid (ABA) and ethylene (ETH) signal transduction. In this study, physiological experiments showed that EBR can increase cold tolerance in cotton seedlings, and the comprehensive RNA-seq data shed light on the mechanisms through which EBR increases cold tolerance in cotton seedlings.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0245070