Genetic characterization of a mild isolate of papaya ringspot virus type-P (PRSV-P) and assessment of its cross-protection potential under greenhouse and field conditions

A mild isolate of Papaya ringspot virus type-P, abbreviated as PRSV-mild, from Ecuador was sequenced and characterized. The most distinguishing symptom induced by PRSV-mild was gray powder-like leaf patches radiating from secondary veins. In greenhouse experiments, PRSV-mild did not confer durable p...

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Bibliographic Details
Published in:PloS one 2021-02, Vol.16 (2), p.e0241652-e0241652
Main Authors: Medina-Salguero, Andres X, Cornejo-Franco, Juan F, Grinstead, Sam, Mowery, Joseph, Mollov, Dimitre, Quito-Avila, Diego F
Format: Article
Language:English
Subjects:
Biodiversity
Biology and Life Sciences
Biopsy
Confocal microscopy
Control
Cross-protection
Disease
Diseases and pests
Disks
Editing
Electron microscopy
Genetic aspects
Genomes
Germplasm
Government regulations
Laboratories
Methodology
Microscopy
Papaya
Papayas
Phylogenetics
Research and Analysis Methods
RNA viruses
Testing
Transmission electron microscopy
Viruses
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Summary:A mild isolate of Papaya ringspot virus type-P, abbreviated as PRSV-mild, from Ecuador was sequenced and characterized. The most distinguishing symptom induced by PRSV-mild was gray powder-like leaf patches radiating from secondary veins. In greenhouse experiments, PRSV-mild did not confer durable protection against a severe isolate of the virus (PRSV-sev), obtained from the same field. Furthermore, isolate specific detection in mixed-infected plants showed that PRSV-sev becomes dominant in infections, rendering PRSV-mild undetectable at 90-120 days post superinfection. Virus testing using isolate-specific primers detected PRSV-mild in two out of five surveyed provinces, with 10% and 48% of incidence in Santo Domingo and Los Ríos, respectively. Comparative genomics showed that PRSV-mild lacks two amino acids from the coat protein region, whereas amino acid determinants for asymptomatic phenotypes were not identified. Recombination events were not predicted in the genomes of the Ecuadorean isolates. Phylogenetic analyses placed both PRSV-mild and PRSV-sev in a clade that includes an additional PRSV isolate from Ecuador and others from South America.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0241652