Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT

Glypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T-3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hed...

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Bibliographic Details
Published in:PloS one 2021-02, Vol.16 (2), p.e0226538-e0226538
Main Authors: Takeuchi, Masao, Takeuchi, Kikuko, Takai, Tomoyo, Yamaguchi, Ritsuko, Furukawa, Tetsushi, Akagi, Ken-Ichi, Takeuchi, Jun K
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Bone morphogenetic proteins
Breast cancer
Cell adhesion & migration
Cell growth
Cell surface
Division
Editing
Epidermal growth factor
Fibroblast growth factor 2
Fibroblast growth factors
G proteins
Gene expression
Genetic aspects
Glioma
Growth factors
Health aspects
Hedgehog protein
Heparan sulfate
Heparan sulfate proteoglycans
Heparin
Heparin-binding epidermal growth factor-like growth factor
Laboratories
Laboratory equipment
Localization
Lung cancer
Magnetic resonance imaging
Mammalian cells
Medical innovations
Medical research
Mesenchymal stem cells
Monoclonal antibodies
Nutrition
Pancreatic cancer
Pharmacology
Proteoglycans
R&D
Receptors
Research & development
Research and Analysis Methods
Signaling
Stem cells
Syndecan
Wnt protein
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Summary:Glypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T-3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hedgehog signaling. In this study, we found using immunofluorescence microscopy that in transformed cells (U3DT), GPC5 localized not only at primary cilia on the cell surface, but also at the leading edge of migrating cells, at the intercellular bridge and blebs during cytokinesis, and in extracellular vesicles. In each subcellular region, GPC5 colocalized with fibroblast growth factor receptor (FGFR) and the small GTPases Rab11 and ARF6, indicating that GPC5 is delivered to these regions by Rab11-associated recycling endosomes. These colocalizations suggest that GPC5 plays an important role in FGF2 stimulation of cell migration, which was abrogated by knockdown of GPC5. Our findings indicate that GPC5 plays a role in regulation of U3DT cell migration and provides several insights into the functions of GPC5 that could be elucidated by future studies.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0226538