Ischemic stroke in PAR1 KO mice: Decreased brain plasmin and thrombin activity along with decreased infarct volume

Ischemic stroke is a common and debilitating disease with limited treatment options. Protease activated receptor 1 (PAR1) is a fundamental cell signaling mediator in the central nervous system (CNS). It can be activated by many proteases including thrombin and plasmin, with various down-stream effec...

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Bibliographic Details
Published in:PloS one 2021-03, Vol.16 (3), p.e0248431-e0248431
Main Authors: Shavit-Stein, Efrat, Mindel, Ekaterina, Gofrit, Shany Guly, Chapman, Joab, Maggio, Nicola
Format: Article
Language:English
Subjects:
Biology and Life Sciences
Care and treatment
Carotid arteries
Coagulation
Deprivation
Disease
Drafting software
Editing
Genetic aspects
Health care facilities
Health risks
Hemorrhage
Ischemia
Laboratory animals
Medical research
Medicine
Medicine and Health Sciences
Methodology
Nervous system
Neurological diseases
Neurology
Nutrients
Oxygen
Pharmacology
Physical Sciences
Physiology
Plasmin
Platelets
Research and Analysis Methods
Reviews
Stroke
Stroke (Disease)
Thrombin
Veins & arteries
Visualization
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Summary:Ischemic stroke is a common and debilitating disease with limited treatment options. Protease activated receptor 1 (PAR1) is a fundamental cell signaling mediator in the central nervous system (CNS). It can be activated by many proteases including thrombin and plasmin, with various down-stream effects, following brain ischemia. A permanent middle cerebral artery occlusion (PMCAo) model was used in PAR1 KO and WT C57BL/6J male mice. Mice were evaluated for neurological deficits (neurological severity score, NSS), infarct volume (Tetrazolium Chloride, TTC), and for plasmin and thrombin activity in brain slices. Significantly low levels of plasmin and thrombin activities were found in PAR1 KO compared to WT (1.6±0.4 vs. 3.2±0.6 ng/μl, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0248431