Genome-wide identification and expression analysis of late embryogenesis abundant protein-encoding genes in rye (Secale cereale L.)

Late embryogenesis abundant (LEA) proteins are members of a large and highly diverse family that play critical roles in protecting cells from abiotic stresses and maintaining plant growth and development. However, the identification and biological function of genes of Secale cereale LEA (ScLEA) have...

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Bibliographic Details
Published in:PloS one 2021-04, Vol.16 (4), p.e0249757-e0249757
Main Authors: Ding, Mengyue, Wang, Lijian, Zhan, Weimin, Sun, Guanghua, Jia, Xiaolin, Chen, Shizhan, Ding, Wusi, Yang, Jianping
Format: Article
Language:English
Subjects:
Abiotic stress
Agronomy
Antioxidants
Barley
Biology and Life Sciences
Chloroplasts
Chromosomes
Computer and Information Sciences
Cytoplasm
Dehydration
Developmental stages
Drought
Ecology and Environmental Sciences
Embryogenesis
Embryonic growth stage
Endoplasmic reticulum
Engineering and Technology
Gene expression
Genes
Genetic aspects
Genomes
Identification and classification
LEA protein
Membrane proteins
Mitochondria
Phylogenetics
Physical Sciences
Plant tissues
Proteins
Rye
Transgenic plants
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Summary:Late embryogenesis abundant (LEA) proteins are members of a large and highly diverse family that play critical roles in protecting cells from abiotic stresses and maintaining plant growth and development. However, the identification and biological function of genes of Secale cereale LEA (ScLEA) have been rarely reported. In this study, we identified 112 ScLEA genes, which can be divided into eight groups and are evenly distributed on all rye chromosomes. Structure analysis revealed that members of the same group tend to be highly conserved. We identified 12 pairs of tandem duplication genes and 19 pairs of segmental duplication genes, which may be an expansion way of LEA gene family. Expression profiling analysis revealed obvious temporal and spatial specificity of ScLEA gene expression, with the highest expression levels observed in grains. According to the qRT-PCR analysis, selected ScLEA genes were regulated by various abiotic stresses, especially PEG treatment, decreased temperature, and blue light. Taken together, our results provide a reference for further functional analysis and potential utilization of the ScLEA genes in improving stress tolerance of crops.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0249757