The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic

Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depl...

Full description

Saved in:
Bibliographic Details
Published in:PLoS genetics 2021-03, Vol.17 (3), p.e1009461-e1009461
Main Authors: Tang, Yajun, Qu, Zhipeng, Lei, Jiajun, He, Reqing, Adelson, David L, Zhu, Youlin, Yang, Zhenbiao, Wang, Dong
Format: Article
Language:English
Subjects:
Analysis
Anthocyanins
Binding sites
Biology and life sciences
Biosynthesis
Cloning
Crops
Fragaria - genetics
Fragaria - growth & development
Fragaria ananassa
Fragaria vesca
Fruit - genetics
Fruits
Gene Editing
Gene expression
Gene Expression Regulation, Plant
Gene silencing
Genes
Genetic Association Studies
Growth
Growth conditions
Identification and classification
Infections
MicroRNAs - genetics
Mimicry (Biology)
Models, Biological
Phenotype
Physiological aspects
Relative humidity
Research and analysis methods
Ripening
RNA
RNA, Guide, CRISPR-Cas Systems
RNA, Long Noncoding
RNA, Messenger - genetics
RNA, Plant
Strawberries
Transcriptomes
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depleted libraries, most of which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved in diverse strawberry species. FRILAIR overexpression promoted fruit maturation in the Falandi strawberry, which was consistent with the finding from knocking down miR397, which can guide the mRNA cleavage of both FRILAIR and LAC11a (encoding a putative laccase-11-like protein). Moreover, LAC11a mRNA levels were increased in both FRILAIR overexpressing and miR397 knockdown fruits, and accelerated fruit maturation was also found in LAC11a overexpressing fruits. Overall, our study demonstrates that FRILAIR can act as a noncanonical target mimic of miR397 to modulate the expression of LAC11a in the strawberry fruit ripening process.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1009461