Infection Manager System (IMS) as a new hemocytometry-based bacteremia detection tool: A diagnostic accuracy study in a malaria-endemic area of Burkina Faso

• Published in: PLoS neglected tropical diseases 2021-03, Vol.15 (3), p.e0009187-e0009187
• Main Authors: Post, Annelies, Kaboré, Berenger, Bognini, Joel, Diallo, Salou, Lompo, Palpouguini, Kam, Basile, Herssens, Natacha, van Opzeeland, Fred, van der Gaast-de Jongh, Christa E, Langereis, Jeroen D, de Jonge, Marien I, Rahamat-Langendoen, Janette, Bousema, Teun, Wertheim, Heiman, Sauerwein, Robert W, Tinto, Halidou, Jacobs, Jan, de Mast, Quirijn, van der Ven, Andre J
• Format: Article
• Language: English
• Subjects: Accuracy; Algorithms; Anaemia; Anemia; Antibiotics; Bacteremia; Bacteria; Bacterial infections; Biology and Life Sciences; Blood; Blood cells; Blood tests; Cell differentiation; Convulsions; Cytodiagnosis; Diagnosis; Differentiation (biology); Erythrocytes; Etiology; Fever; Haematology; Health; Hematology; Hemoglobin; Human diseases; Illnesses; Infections; Inflammation; Inflammatory response; Jaundice; Malaria; Malnutrition; Medical examination; Medicine and Health Sciences; Methods; Pathogens; Prototypes; Red blood cells; Software; Symptoms; Tropical diseases; Vector-borne diseases; Viral infections
• ISSN: 1935-2735; 1935-2727; 1935-2735
• DOI: 10.1371/journal.pntd.0009187

New hemocytometric parameters can be used to differentiate causes of acute febrile illness (AFI). We evaluated a software algorithm-Infection Manager System (IMS)-which uses hemocytometric data generated by Sysmex hematology analyzers, for its accuracy to detect bacteremia in AFI patients with and without malaria in Burkina Faso. Secondary aims included comparing the accuracy of IMS with C-reactive protein (CRP) and procalcitonin (PCT). In a prospective observational study, patients of ≥ three-month-old (range 3 months- 90 years) presenting with AFI were enrolled. IMS, blood culture and malaria diagnostics were done upon inclusion and additional diagnostics on clinical indication. CRP, PCT, viral multiplex PCR on nasopharyngeal swabs and bacterial- and malaria PCR were batch-tested retrospectively. Diagnostic classification was done retrospectively using all available data except IMS, CRP and PCT results. A diagnosis was affirmed in 549/914 (60.1%) patients and included malaria (n = 191) bacteremia (n = 69), viral infections (n = 145), and malaria-bacteremia co-infections (n = 47). The overall sensitivity, specificity, and negative predictive value (NPV) of IMS for detection of bacteremia in patients of ≥ 5 years were 97.0% (95% CI: 89.8-99.6), 68.2% (95% CI: 55.6-79.1) and 95.7% (95% CI: 85.5-99.5) respectively, compared to 93.9% (95% CI: 85.2-98.3), 39.4% (95% CI: 27.6-52.2), and 86.7% (95% CI: 69.3-96.2) for CRP at ≥20mg/L. The sensitivity, specificity and NPV of PCT at 0.5 ng/ml were lower at respectively 72.7% (95% CI: 60.4-83.0), 50.0% (95% CI: 37.4-62.6) and 64.7% (95% CI: 50.1-77.6) The diagnostic accuracy of IMS was lower among malaria cases and patients