Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources

• Published in: PloS one 2021-05, Vol.16 (5), p.e0251456-e0251456
• Main Authors: Tuong Vi, Dang Thi, Fujii, Shiori, Valderrama, Arvin Lapiz, Ito, Ayaka, Matsuura, Eri, Nishihata, Ayaka, Irie, Kaoru, Suda, Yasuyuki, Mizuno, Tomoaki, Irie, Kenji
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Ataxin; Biology; Biology and Life Sciences; Carbon; Carbon sources; Cell growth; Cellular stress response; Data analysis; Deoxyribonucleic acid; Dextrose; DNA; DNA microarrays; Editing; Energy metabolism; Gene expression; Gene regulation; Genes; Genetic aspects; Genetic translation; Glucose; Glycerol; Graduate schools; Graduate studies; Image resolution; Information flow; Introns; Kinases; Medicine; Metabolism; Methodology; Plasmids; Polyadenine; Polyadenylation; Post-transcription; Proteins; Research and Analysis Methods; Reviews; Visualization; Yeast; Yeast fungi; Yeasts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0251456

Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. In this study, we found that Pbp1 has a role in cell growth on the medium containing non-fermentable carbon sources. While the pbp1Δ mutant showed a similar growth compared to the wild-type cell on a normal glucose-containing medium, the pbp1Δ mutant showed a slower growth on the medium containing glycerol and lactate. Microarray analyses revealed that expressions of the genes involved in gluconeogenesis, such as PCK1 and FBP1, and of the genes involved in mitochondrial function, such as COX10 and COX11, were decreased in the pbp1Δ mutant. Pbp1 regulated the expressions of PCK1 and FBP1 via their promoters, while the expressions of COX10 and COX11 were regulated by Pbp1, not through their promoters. The decreased expressions of COX10 and COX11 in the pbp1Δ mutant were recovered by the loss of Dcp1 decapping enzyme or Xrn1 5'-3'exonuclease. Our results suggest that Pbp1 regulates the expressions of the genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms.