Neuraminidase B controls neuraminidase A-dependent mucus production and evasion

• Published in: PLoS pathogens 2021-04, Vol.17 (4), p.e1009158
• Main Authors: Hammond, Alexandria J, Binsker, Ulrike, Aggarwal, Surya D, Ortigoza, Mila Brum, Loomis, Cynthia, Weiser, Jeffrey N
• Format: Article
• Language: English
• Subjects: Agar; Animals; Antibodies; Bacteria; Bacterial Proteins - metabolism; Biological Transport - drug effects; Biology and Life Sciences; Development and progression; Enzymes; Exo-a-sialidase; Experiments; Glycoside Hydrolases - drug effects; Glycoside Hydrolases - metabolism; Health aspects; Hydrolases; Medicine and Health Sciences; Mice; Mice, Inbred C57BL; Microscopy; Mucus; N-Acetylneuraminic Acid - metabolism; N-Acetylneuraminic Acid - pharmacology; Nasal mucosa; Neuraminidase - metabolism; Neuraminidase - pharmacology; Physical Sciences; Physiological aspects; Plates; Plating; Polysaccharides; Research and analysis methods; Saccharides; Streptococcal infections; Streptococcus infections; Streptococcus pneumoniae - drug effects; Streptococcus pneumoniae - metabolism; Streptomycin
• ISSN: 1553-7374; 1553-7366; 1553-7374
• DOI: 10.1371/journal.ppat.1009158

Binding of Streptococcus pneumoniae (Spn) to nasal mucus leads to entrapment and clearance via mucociliary activity during colonization. To identify Spn factors allowing for evasion of mucus binding, we used a solid-phase adherence assay with immobilized mucus of human and murine origin. Spn bound large mucus particles through interactions with carbohydrate moieties. Mutants lacking neuraminidase A (nanA) or neuraminidase B (nanB) showed increased mucus binding that correlated with diminished removal of terminal sialic acid residues on bound mucus. The non-additive activity of the two enzymes raised the question why Spn expresses two neuraminidases and suggested they function in the same pathway. Transcriptional analysis demonstrated expression of nanA depends on the enzymatic function of NanB. As transcription of nanA is increased in the presence of sialic acid, our findings suggest that sialic acid liberated from host glycoconjugates by the secreted enzyme NanB induces the expression of the cell-associated enzyme NanA. The absence of detectable mucus desialylation in the nanA mutant, in which NanB is still expressed, suggests that NanA is responsible for the bulk of the modification of host glycoconjugates. Thus, our studies describe a functional role for NanB in sialic acid sensing in the host. The contribution of the neuraminidases in vivo was then assessed in a murine model of colonization. Although mucus-binding mutants showed an early advantage, this was only observed in a competitive infection, suggesting a complex role of neuraminidases. Histologic examination of the upper respiratory tract demonstrated that Spn stimulates mucus production in a neuraminidase-dependent manner. Thus, an increase production of mucus containing secretions appears to be balanced, in vivo, by decreased mucus binding. We postulate that through the combined activity of its neuraminidases, Spn evades mucus binding and mucociliary clearance, which is needed to counter neuraminidase-mediated stimulation of mucus secretions.