Bioconversion of potato solid waste into antifungals and biopigments using Streptomyces spp

• Published in: PloS one 2021-05, Vol.16 (5), p.e0252113-e0252113
• Main Authors: Schalchli, Heidi, Hormazábal, Emilio, Astudillo, Álvaro, Briceño, Gabriela, Rubilar, Olga, Diez, María Cristina
• Format: Article
• Language: English
• Subjects: Agribusiness; Agricultural production; Agricultural wastes; Agriculture; Antibiotics; Antifungal agents; Bacteria; Bioconversion; Biology and Life Sciences; Biotechnology; Carbohydrates; Chemical composition; Chemical engineering; Chemical properties; Editing; Electronic mail; Environments; Feeds; Food; Food consumption; Food processing industry; Funding; Gram-positive bacteria; Industrial wastes; Medicine and Health Sciences; Methods; Microorganisms; Morphology; Natural resources; Pathogens; Physical Sciences; Pigments; Potatoes; R&D; Refuse and refuse disposal; Research & development; Research and Analysis Methods; Research facilities; Scanning electron microscopy; Solid wastes; Spectroscopy; Spectrum analysis; Supply chains; Waste disposal; X-ray spectroscopy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0252113

Potato waste was processed and used as a sole substrate for simultaneously producing antifungals and biopigments using Streptomyces spp. Out of three different Streptomyces isolates, strain SO6 stood out due to its ability to produce antifungals against economically important fungal phytopathogens and intracellular biopigments using potato waste powders without additional nutrients. This strain also showed the potential to secrete a broad range of enzymes for fermentation of eight sugars that could be involved in potato waste bioconversion. The results of the fermentation assay indicated that Streptomyces sp. strain SO6 degrades potato wastes during submerged fermentation, diminishing total dry weight and increasing reducing sugars from 0.3 to 3.6 mg·mL.sup.-1 and total proteins from 70.6 to 187.7 [mu]g·mL.sup.-1 . The results showed that Streptomyces strain SO6 was able to convert the potato waste into 0.96 mg·g.sup.-1 of diffusible antifungals and 1.75 mg·g.sup.-1 of reddish-purple biopigments. On the contrary, an absence of pigment production was observed during the fermentation of the commercial medium used as reference. According to our results, replacement of commercial culture media with available low-cost agroindustrial wastes for producing bioactive chemicals is a real opportunity to enhance the Streptomyces pigment production and antibiotic sustainability with cost-competitiveness. To our knowledge, this is the first report on the simultaneous production of biopigments and diffusible antifungal antibiotics produced by Streptomyces spp. using potato solid waste as the sole nutrient source.