Herb-partitioned moxibustion alleviates colonic inflammation in Crohn’s disease rats by inhibiting hyperactivation of the NLRP3 inflammasome via regulation of the P2X7R-Pannexin-1 signaling pathway

• Published in: PloS one 2021-05, Vol.16 (5), p.e0252334-e0252334
• Main Authors: Zhang, Ji, Wang, Xue-jun, Wu, Li-jie, Yang, Ling, Yang, Yan-ting, Zhang, Dan, Hong, Jue, Li, Xi-ying, Dong, Xiao-qing, Guo, Xiao-cong, Han, Rong, Ma, Xiaopeng
• Format: Article
• Language: English
• Subjects: Acupuncture; Animal welfare; Apoptosis; Assaying; ATP; Biological activity; Biology and Life Sciences; Caspase-1; Chinese medicine; Chloroform; Clinical medicine; Colitis; Colon; Complications and side effects; Crohn's disease; Cytokines; Disease; Ethanol; Ethical standards; Ethics; Genetic aspects; Glyceraldehyde-3-phosphate dehydrogenase; Herbs; Hydrogen; IL-1β; Immunoglobulin G; Immunology; Inflammasomes; Inflammation; Inflammatory bowel diseases; Interleukin 18; Intestine; Laboratory animals; Lysis; Medical research; Medicine and Health Sciences; Monoclonal antibodies; Moxibustion; Odor; Odors; Pathogenesis; Patient outcomes; Pentobarbital; Pentobarbital sodium; Polyclonal antibodies; Reagents; Research and Analysis Methods; Signal transduction; Signaling; Stem cells; Sulfonic acid; Technology; Xylene
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0252334

Crohn's disease is a chronic inflammatory bowel disease and the NLRP3 inflammasome plays an important role in Crohn's disease. Previous studies have shown that Herb-partitioned moxibustion treating (at Qihai (CV 6) and Tianshu (ST 25)) prevented the excessive activation of the NLRP3 inflammasome and repaired damaged colonic mucosa in Crohn's disease. However, the mechanism by which Herb-partitioned moxibustion (at CV 6 and ST 25) regulates NLRP3 remains unclear. In this study, we treated Crohn's disease rats with herb-partitioned moxibustion (at CV 6 and ST 25) to investigate the mechanism by which Herb-partitioned moxibustion regulates the colonic NLRP3 inflammasome by observing colon length, the colon macroscopic damage indexes, and the expression of ATP, P2X7R, Pannexin-1, NF-[kappa]Bp65, NLRP3, ASC, caspase-1, IL-1[beta] and IL-18 in the colon in Crohn's disease. Here, this study shows that herb-partitioned moxibustion (at CV 6 and ST 25) can reduce colon macroscopic damage indexes and colon histopathological scores, alleviate colon shortening and block the abnormal activation of the NLRP3 inflammasome by inhibiting the ATP content and the expression of P2X7R, Pannexin-1 and NF-[kappa]Bp65, thereby reducing the release of the downstream inflammatory cytokine IL-1[beta] and ultimately suppressing colonic inflammation in Crohn's disease rats. This study for the first time identifies the mechanism by which herb-partitioned moxibustion (at CV 6 and ST 25) may inhibit the abnormal activation of the NLRP3 inflammasome by inhibiting the P2X7R-Pannexin-1 signaling pathway in Crohn's disease rats.