Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi

• Published in: PloS one 2021-06, Vol.16 (6), p.e0252214
• Main Authors: Hove, Petronella R, Magunda, Forgivemore, de Mello Marques, Maria Angela, Islam, M Nurul, Harton, Marisa R, Jackson, Mary, Belisle, John T
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacterial proteins; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biology and Life Sciences; Borrelia burgdorferi; Borrelia burgdorferi - genetics; Borrelia burgdorferi - metabolism; Cell density; Cholesterol; Cholesterol - metabolism; Cloning; Development and progression; E coli; Editing; Enzymes; Functional analysis; Galactosides; Galactosyltransferases - genetics; Galactosyltransferases - metabolism; Gene expression; Glycolipids; Glycolipids - metabolism; Health aspects; Homology; Humans; Immunology; Laboratories; Lipids; Lyme disease; Lyme Disease - microbiology; Medicine and Health Sciences; Methodology; Microbiological research; Microbiology; Microscopy; Pathology; Physiological aspects; Proteins; Reaction products; Reviews; Spirochetes; Stereochemistry; Substrates; Synthesis; Transferases; Vector-borne diseases
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0252214

Borrelia burgdorferi (Bb), the etiological agent of Lyme disease, produces a series of simple glycolipids where diacylglycerol and cholesterol serve as the precursor. The cholesterol-based glycolipids, cholesteryl 6-O-acyl-β-D-galactopyranoside (ACGal) and cholesteryl-β-D-galactopyranoside (CGal) are immunogenic and proposed to contribute to the pathogenesis of Lyme disease. Detailed studies of CGal and ACGal in Bb have been hampered by a lack of knowledge of their underlying biosynthetic processes. The genome of Bb encodes four putative glycosyltransferases, and only one of these, BB0572, was predicted to be an inverting family 2 glycosyltransferase (GT2 enzyme) capable of using UDP-galactose as a substrate and forming a β-glycosidic bond. Comparison of the 42 kDa BB0572 amino acid sequence from Bb with other Borrelia spp demonstrates that this protein is highly conserved. To establish BB0572 as the galactosyltransferase capable of cholesterol glycolipid formation in Bb, the protein was produced as a recombinant product in Escherichia coli and tested in a cell-free assay with 14C-cholesterol and UDP-galactose as the substrates. This experiment resulted in a radiolabeled lipid that migrated with the cholesterol glycolipid standard of CGal when evaluated by thin layer chromatography. Additionally, mutation in the predicted active site of BB0572 resulted in a recombinant protein that was unable to catalyze the formation of the cholesterol glycolipid. These data characterize BB0572 as a putative cholesterol galactosyltransferase. This provides the first step in understanding how Bb cholesterol glycolipids are formed and will allow investigations into their involvement in pathogen transmission and disease development.