Novel low shear 3D bioreactor for high purity mesenchymal stem cell production

Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a r...

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Bibliographic Details
Published in:PloS one 2021-06, Vol.16 (6), p.e0252575-e0252575
Main Authors: Burns, Andrew B, Doris, Corinna, Vehar, Kevin, Saxena, Vinit, Bardliving, Cameron, Shamlou, Parviz A, Phillips, M. Ian
Format: Article
Language:English
Subjects:
3-D printers
3D printing
Biology and Life Sciences
Bioreactors
Cell culture
Cell growth
Cell proliferation
Chronic illnesses
Design
Design and construction
Drug dosages
Engineering and technology
Feasibility studies
Fixed bed reactors
Gene therapy
Hypoxia
Life sciences
Medical research
Medicine and Health Sciences
Mesenchymal stem cells
Methods
Physical Sciences
Purity
Regenerative medicine
Research and Analysis Methods
Scaffolds
Shear stress
Stainless steel
Stem cell research
Stem cells
Three dimensional printing
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Summary:Bone marrow derived human Mesenchymal Stem Cells (hMSCs) are an attractive candidate for regenerative medicine. However, their harvest can be invasive, painful, and expensive, making it difficult to supply the enormous amount of pure hMSCs needed for future allogeneic therapies. Because of this, a robust method of scaled bioreactor culture must be designed to supply the need for high purity, high density hMSC yields. Here we test a scaled down model of a novel bioreactor consisting of an unsubmerged 3D printed Polylactic Acid (PLA) lattice matrix wetted by culture media. The growth matrix is uniform, replicable, and biocompatible, enabling homogenous cell culture in three dimensions. The goal of this study was to prove that hMSCs would culture well in this novel bioreactor design. The system tested resulted in comparable stem cell yields to other cell culture systems using bone marrow derived hMSCs, while maintaining viability (96.54% ±2.82), high purity (>98% expression of combined positive markers), and differentiation potential.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0252575