Age- and sex-associated differences in hematology and biochemistry parameters of Dunkin Hartley guinea pigs (Cavia porcellus)

• Published in: PloS one 2021-07, Vol.16 (7), p.e0253794-e0253794
• Main Authors: Spittler, Alexa P, Afzali, Maryam F, Bork, Sydney B, Burton, Lindsey H, Radakovich, Lauren B, Seebart, Cassie A, Moore, A. Russell, Santangelo, Kelly S
• Format: Article
• Language: English
• Subjects: Age; Alanine; Alanine transaminase; Albumin; Albumins; Alkaline phosphatase; Allergies; Analysis; Animal experimentation; Anions; Aspartate aminotransferase; Asthma; Bicarbonates; Biochemistry; Biology and Life Sciences; Biomedical materials; Blood; Blood levels; Calcium (blood); Catheters; Cholesterol; Confidence intervals; Creatinine; Eosinophils; Erythrocytes; Evaluation; Females; Gender aspects; Globulins; Guinea pigs; Hematocrit; Hematology; Hemoglobin; Hypersensitivity; Immunology; Infectious diseases; Intervals; Laboratory animals; Leukocytes (eosinophilic); Leukocytes (neutrophilic); Lymphocytes; Magnesium; Males; Mathematical analysis; Medicine and Health Sciences; Osteoarthritis; Parameters; Pathology; Phosphorus; Physical Sciences; Platelets; Potassium; Proteins; Research and Analysis Methods; Sex; Sex differences; Swine; Urea; Veterinary colleges; Veterinary medicine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0253794

The Dunkin Hartley is the most common guinea pig strain used in biomedical research, particularly for studies of asthma, allergy, infectious disease, reproduction, and osteoarthritis. Minimally invasive blood tests, such as complete blood counts and serum biochemistry profiles, are often collected for diagnostics and laboratory analyses. However, reference intervals for these assays have not yet been well-documented in this strain. The purpose of this study was to establish reference intervals for hematologic and biochemical parameters of Dunkin Hartley guinea pigs and determine age- and sex-related differences. Hematologic and biochemical parameters were retrospectively obtained from 145 male and 68 female guinea pigs between 2 and 15 months of age. All blood parameters were analyzed by a veterinary clinical pathology laboratory. Reference intervals were established according to the American Society for Veterinary Clinical Pathology guidelines. Age- and sex-related differences were determined using unpaired t-tests or nonparametric Mann-Whitney tests. Hematocrit, red blood cell distribution width, mean platelet volume, white blood cell count, heterophils, monocytes, eosinophils, glucose, blood urea nitrogen, creatinine, calcium, magnesium, total protein, albumin, globulin, cholesterol, aspartate aminotransferase, gamma glutamyl transferase, and bicarbonate increased with age. Mean corpuscular hemoglobin concentration, cellular hemoglobin concentration mean, platelets, lymphocytes, phosphorus, albumin/globulin ratio, alkaline phosphatase, anion gap, and calculated osmolality decreased with age. Males had higher hemoglobin, hematocrit, red blood cell count, mean corpuscular hemoglobin concentration, white blood cell count, heterophils, Foa-Kurloff cells, alanine aminotransferase, and bicarbonate and lower mean corpuscular volume, red blood cell distribution width, platelets, mean platelet volume, eosinophils, total protein, albumin, globulin, cholesterol, potassium, anion gap, calculated osmolality, and iron compared to females. Establishing age and sex differences in hematologic and biochemical parameters of Dunkin Hartley guinea pigs provides valuable insight into their physiology to better evaluate diagnostics and experimental results.