Development and analytical validation of a novel bioavailable 25-hydroxyvitamin D assay

Bioavailable 25-hydroxyvitamin D (25OHD) may be a better indicator of vitamin D sufficiency than total 25OHD. This report describes a novel assay for measuring serum bioavailable 25OHD. We developed an assay for 25OHD % bioavailability based on competitive binding of 25OHD tracer between vitamin D-b...

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Published in:PloS one 2021-07, Vol.16 (7), p.e0254158
Main Authors: Berg, Anders H, Tavasoli, Mahtab, Lo, Agnes S, Burnett-Bowie, Sherri-Ann M, Bhan, Ishir, Karumanchi, S Ananth, Kalim, Sahir, Zhang, Dongsheng, Zhao, Sophia, Thadhani, Ravi I
Format: Article
Language:English
Subjects:
25-Hydroxyvitamin D
Adult
Affinity chromatography
Aged
Albumins
Alfacalcidol
Assaying
Beads
Bioavailability
Biological assay
Biological Availability
Biology
Biology and Life Sciences
Calcifediol
Calciferol
Case-Control Studies
Chromatography
Chromatography, Affinity - methods
DBP protein
Equilibrium
Equivalence
Female
Health aspects
Homeostasis
Humans
Hypotheses
Interpolation
Male
Mathematical analysis
Measurement
Medical research
Medical schools
Medicine
Medicine and Health Sciences
Medicine, Experimental
Methods
Middle Aged
Nephrology
Patients
Physical sciences
Proteins
Research and Analysis Methods
Vitamin D
Vitamin D - analogs & derivatives
Vitamin D - blood
Vitamin D-binding protein
Vitamin D-Binding Protein - blood
Vitamin D-Binding Protein - metabolism
Vitamin deficiency
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Summary:Bioavailable 25-hydroxyvitamin D (25OHD) may be a better indicator of vitamin D sufficiency than total 25OHD. This report describes a novel assay for measuring serum bioavailable 25OHD. We developed an assay for 25OHD % bioavailability based on competitive binding of 25OHD tracer between vitamin D-binding protein (DBP)-coated affinity chromatography beads and serum DBP. Bioavailable 25OHD, total 25OHD, albumin, and DBP protein concentrations were measured in 89 samples from hospitalized patients and 42 healthy controls to determine how the DBP binding assay responds to differences in concentrations of DBP and compares to calculated bioavailable 25OHD values. DBP binding assay showed a linear relationship between DBP-bound 25OHD tracer recovered from bead supernatant and DBP calibrator concentrations (y = 0.0017x +0.731, R2 = 0.9961, p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0254158