Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles

The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical par...

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Bibliographic Details
Published in:PloS one 2021-07, Vol.16 (7), p.e0255378-e0255378
Main Authors: Manukhova, Tatiana I, Evtushenko, Ekaterina A, Ksenofontov, Alexander L, Arutyunyan, Alexander M, Kovalenko, Angelina O, Nikitin, Nikolai A, Karpova, Olga V
Format: Article
Language:English
Subjects:
Adsorption
Alternanthera
Amino acids
Biochemical analysis
Biology
Biology and Life Sciences
Circular Dichroism
Conformation
Dichroism
Fluorescence
Fluorescence spectroscopy
Labeling
Medicine and Health Sciences
Microscopy
Microscopy, Electron, Transmission
Nanoparticles
Nicotiana - virology
Physical Sciences
Physiological aspects
Plant viruses
Potexvirus - genetics
Potexvirus - physiology
Potexviruses
Proteins
Research and analysis methods
RNA, Viral - chemistry
RNA, Viral - isolation & purification
RNA, Viral - metabolism
Spectrometry, Fluorescence
Spectroscopy
Temperature
Virion - chemistry
Virion - physiology
Virions
Virology
Virus research
Virus-like particles
Viruses
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Summary:The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0255378