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Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles
The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical par...
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Published in: | PloS one 2021-07, Vol.16 (7), p.e0255378-e0255378 |
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description | The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes. |
doi_str_mv | 10.1371/journal.pone.0255378 |
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Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>34320024</pmid><doi>10.1371/journal.pone.0255378</doi><tpages>e0255378</tpages><orcidid>https://orcid.org/0000-0001-9786-7652</orcidid><orcidid>https://orcid.org/0000-0001-9626-2336</orcidid><orcidid>https://orcid.org/0000-0001-9498-1257</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Adsorption Alternanthera Amino acids Biochemical analysis Biology Biology and Life Sciences Circular Dichroism Conformation Dichroism Fluorescence Fluorescence spectroscopy Labeling Medicine and Health Sciences Microscopy Microscopy, Electron, Transmission Nanoparticles Nicotiana - virology Physical Sciences Physiological aspects Plant viruses Potexvirus - genetics Potexvirus - physiology Potexviruses Proteins Research and analysis methods RNA, Viral - chemistry RNA, Viral - isolation & purification RNA, Viral - metabolism Spectrometry, Fluorescence Spectroscopy Temperature Virion - chemistry Virion - physiology Virions Virology Virus research Virus-like particles Viruses |
title | Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles |
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