Role of nearshore benthic algae in the Lake Michigan silica cycle

• Published in: PloS one 2021-08, Vol.16 (8), p.e0256838-e0256838
• Main Authors: Berges, John A, Driskill, Allison M, Guinn, Emily J, Pokrzywinski, Kaytee, Quinlan, Jessica, von Korff, Benjamin, Young, Erica B
• Format: Article
• Language: English
• Subjects: Algae; Alkaline digestion; Aquatic ecosystems; Benthos (Aquatic organisms); Biogeochemistry; Biology and Life Sciences; Biomass; Carbon sequestration; Cladophora; Coastal waters; Colorimetry; Community composition; Cycles; Diatoms; Diatoms - metabolism; Earth Sciences; Ecology and Environmental Sciences; Ecosystem; Environmental aspects; Environmental Monitoring; Epiphytes; Eutrophication; Growth rate; Humans; Incorporation; Lakes; Localization; Marine microorganisms; Michigan; Mollusks; Plankton; Scanning electron microscopy; Seaweeds; Sediments; Silica; Silicon dioxide; Silicon Dioxide - metabolism; Water circulation; Water column
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0256838

Si cycling is linked with processes from global carbon sequestration to community composition and is especially important in aquatic ecosystems. Lake Michigan has seen dramatic fluctuations in dissolved silica (dSi) over several decades, which have been examined in the context of planktonic processes (diatom blooms), but the role of benthic organisms (macroalgae and their epiphytes) in Si cycling have not been explored. To assess significance of nearshore benthic algae in Si dynamics, we assembled dSi data from an offshore site sampled since the late 1980's, and sampled off three Milwaukee beaches during 2005-19. Using colorimetric assays and alkaline digestion, we measured dSi, biogenic silica in particulate suspended material (pSi) and biogenic silica in benthic macroalgae (Cladophora) and epiphytic diatoms (bSi). Offshore, dSi increased about 1 μM per year from 25 μM in the late 1980's to nearly 40 μM in 2019. Nearshore dSi fluctuated dramatically annually, from near zero to concentrations similar to offshore. Both Cladophora and its epiphytes contained significant bSi, reaching up to 30% of dry mass (300 mg Si g dry mass-1) of the assemblage in summer. Microscopic analyses including localization with a Si-specific-stain and X-ray microanalysis showed bSi in epiphytic diatom cells walls, but the nature and localization of Si in macroalgae remained unclear. A simple model was developed estimating Si demand of algae using the areal macroalgal biomass, growth rates inferred from P-content, and bSi content, and comparing Si demand with dSi available in the water column. This indicated that 7-70% of the dSi in water overlying nearshore benthic algal beds could be removed per day. Key elements of the Si cycle, including which organisms sequester bSi and how rapidly Si is recycled, remain unclear. This work has implications for coastal marine waters where large macroalgal biomass accumulates but bSi content is virtually unknown.